细胞电参数对内外膜跨膜电位影响的仿真研究  

Simulation of Transmembrane Potentials on Inner and Outer Membrane under Different Cellular Electrical Parameters

作　　者：姚陈果[1] 莫登斌[1] 孙才新[1] 米彦[1] 李成祥[1] 

机构地区：[1]重庆大学电气工程学院高电压与电工新技术教育部重点实验室,重庆400044

出　　处：《中国生物医学工程学报》2007年第5期739-745,共7页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金项目(50637020;50407007);教育部博士点基金(20050611020);教育部新世纪优秀人才支持计划(NCET-06-0768);重庆市科委科技项目(2005AA5008-3;2005BB5251)。

摘　　要：基于球形单细胞多层介电模型,提出了细胞电参数对内外膜跨膜电位影响的计算方法。仿真分析了细胞膜、细胞质、核膜、细胞核质的电导率和介电常数对内外膜跨膜电位的影响。结果表明:细胞膜跨膜电位主要受细胞质电导率和细胞膜介电常数影响,细胞质电导率越大,细胞膜介电常数越小,细胞膜跨膜电位就越大;核膜跨膜电位主要受细胞质和核质电导率、核膜介电常数的影响,细胞质和核质电导率越大,核膜介电常数越小,核膜跨膜电位越大。仿真结果为确定细胞内电处理肿瘤疗法的适应症提供了一定的理论指导。Based on multi-shelled dielectric model of spherical cell, calculation method for the influence of cellular parameters on transmembrane potentials on inner and outer membrane was presented. The influence of conductivity and dielectric permittivity of cytoplasm, nuclear matrix, outer and inner membrane on transmembrane potentials on inner and outer membrane was simulated. The results revealed that the transmembrane potential on outer membrane was mainly affected by cytoplasmic conductivity and dielectric permittivity of outer membrane. Higher cytoplasmic conductivity or lower dielectric permittivity of outer membrane resulted in a higher transmembrane potential on outer membrane. However, transmembrane potential on inner membrane was mainly related to conductivity of cytoplasmic and nuclear matrix and dielectric permittivity of inner membrane. Furthermore, the transmembrane potential on inner membrane was increased with the cytoplasmic conductivity or nuclear matrix conductivity proportionally, and decreased with dielectric permittivity of inner membrane. The simulation results provided a reasonable theoretical guidance to determine the indication of applying intracellular electromanipulation to treat tumor.

关 键 词：跨膜电位 电参数 影响 适应症 

分 类 号：Q64[生物学—生物物理学]