免疫抑制宿主巨噬细胞Toll样受体的表达变化  

Change of toll-like receptor mRNA expression in peritoneal macrophages of immunosuppressed mice

作　　者：顾克菊[1] 马越云[1] 苏明权[1] 郝晓柯[1] 于文彬[1] 

机构地区：[1]第四军医大学西京医院检验科,陕西西安710033

出　　处：《第四军医大学学报》2007年第20期1888-1890,共3页Journal of the Fourth Military Medical University

摘　　要：目的:检测小鼠腹腔巨噬细胞TLRs表达情况及糖皮质激素甲基强的松龙对TLRsmRNA表达的影响.方法:应用RT-PCR检测正常小鼠腹腔巨噬细胞TLR1-13的表达;并以甲基强地松龙为免疫抑制剂,诱导小鼠免疫抑制后检测腹腔巨噬细胞TLRsmRNA表达变化.结果:已发现的TLR1-13在正常小鼠腹腔巨噬细胞均有表达;小鼠腹腔巨噬细胞经糖皮质激素处理后,部分TLRs在mRNA水平上可被上调.结论:Toll样受体是抗真菌先天免疫的重要组成部分.甲基强的松龙对巨噬细胞有毒性作用,甲基强的松龙注射后可导致小鼠腹腔巨噬细胞明显减少.AIM： To test the expression of toll-like receptor 1- 13 （TLRI-13） mRNA in the peritoneal maerophages of normal mice and the change of TLRs mRNA expression in peritoneal macrophages of immunosuppressed mice. METHODS： The expression of TLR1-13 mRNA in the peritoneal maerophages of normal mice was determined by RT-PCR technique. Immunosuppressed mice were induced by intraperitoneal injections of prednisolone. The expression of TLRs mRNA in the peritoneal maerophages of immunosuppressed mice was tested with RT-PCR. RESULTS： All thirteen of the TLRs reported to date were present in the maerophages of normal mice. Some of TLRs were up-regulated in mRNA level when the mice were treated with glueocortieoids. CONCLUSION： TLRs are important component parts of innate immunity to fungal infections. Prednisolone has a toxic effect to maerophages and decreases the peritoneal maerophages of the mice.

关 键 词：小鼠 泼尼松龙 免疫抑制法 TOLL样受体 

分 类 号：R392[医药卫生—免疫学]