纤维蛋白原与吸附白蛋白、肝素的新型血管支架材料氧化钛的血液相容性  

Study on a Novel Vascular Stent Material(Titanium Oxide,Ti-O) Coated with Albumin and Heparin:Is It Hemocompatible with Fibrinogen

作　　者：宋杰[1] 吴熹[2] 黄楠[2] 徐标[1] 景凤娟[2] 陈俊英[2] 冷永祥[2] 杨苹[2] 王进[2] 孙鸿[2] 赵安莎[2] 

机构地区：[1]南京大学医学院附属鼓楼医院心脏科,南京210008 [2]西南交通大学材料学院,成都610031

出　　处：《生物医学工程学杂志》2007年第5期1097-1101,共5页Journal of Biomedical Engineering

基　　金：国家重点基础研究规划项目资助(G1999064706);国家自然科学基金资助项目(30318006;39470200)

摘　　要：探索纤维蛋白原(Fibrinogen,FIG)与吸附白蛋白、肝素的新型血管支架材料氧化钛(Titanium Oxide,Ti-O)的血液相容性。(1)研制Ti-O,切割成薄膜;(2)Ti-O薄膜涂层白蛋白和肝素;(3)血小板(platelet,PL)吸附试验;(4)酶联免疫试验测FIG吸附量;(5)动物犬股动脉内植入涂层的Ti-O薄膜与对照试片Ti-O和不锈钢(Stainless steel,SS)薄膜。结果发现:Ti-O完全具有固定白蛋白和肝素的结构与性能,比未涂层的Ti-O能更一步减少PL和FIG的吸附,实验动物体内薄膜6个月后取出扫描电镜观察黏附的PL少,形态无改变,血管内无血栓,优于未涂层的Ti-O,更明显优于SS。Ti-O为N型半导体,不易接受FIG的电荷,并且与血细胞有相似的界面张力,决定生物材料Ti-O有较好的血液相容性。Ti-O对白蛋白、肝素有极好的亲和力是因以化学键相结合,在血中进一步减少FIG和PL被涂层的Ti-O吸附。实验证明涂层的Ti-O有持久和稳定的抗凝血性能。The functional hemocompatibility between fibrinogen （FIG） and a novel vascular stent material （Ti-O film fixed with albumin and heparin） was investigated as follows： （1） Preparing the new biologic material （Ti-O） film; （2） Coating albumin and heparin on the Ti-O film;（3） Testing platelets （PL） adsorption; （4） Determining FIG adhesion number by use of enzyme linked immunoassay （ELISA） ; （5） Implanting the films from the test group of Ti-O film and from the comparison group of stainless steel（SS） film into the left and right femoral arteries respectively in 4 dogs. It was proved that albumin and heparin were fixed on Ti-O film. After 6 months, the femoral arteries of the dogs were resected. In the test group of Ti-O film coated with albumin and heparin, few PL adhered to the coat, their form did not change, and no thrombus was found by scanning electron microscopy; the result was better than that of plain Ti-O film, and was much better than that of SS film. Ti-O maintained normal transformation condition of FIG, and no C terminal of γ chain in FIG was revealed. As it is known whether the hemocompatibility of a biomaterial is good depends upon its adsorption of FIG, and Ti-O has excellent reaction on albumin and heparin by chemical processes. In this study, the Ti-O film coated with albumin and heparin further reduced the absorption of FIG and PL when compared against the plain Ti-O film. So the Ti-O film coated with albumin and heparin has the insistent and permanent anticoagulant character.

关 键 词：白蛋白 肝素 血管支架 血液相容性 

分 类 号：R318.08[医药卫生—生物医学工程]