原子力显微镜在DNA和蛋白质相互作用方面的研究进展  

Progress in the Studies of DNA-protein Interactions by Atomic Force Microscopy

作　　者：王云起[1] 廖问陶[1] 蔡继业[1] 

机构地区：[1]暨南大学生命科学技术学院化学系,广州510632

出　　处：《生物医学工程学杂志》2007年第5期1172-1176,共5页Journal of Biomedical Engineering

基　　金：国家重点基础研究发展规划项目(973)资助(2001CB510101);国家自然科学基金资助项目(重点项目30230350和面上项目60278014)

摘　　要：评述了原子力显微镜在研究DNA和蛋白质相互作用领域的应用进展。AFM可在空气和液体中对静态的DNA-protein复合体进行成像,也可在液体中实时观察DNA和protein的反应过程。而且,力模式AFM还可获得DNA和protein分子间作用力的信息。AFM已揭示了许多基因调控的机制,也必将在生命科学的研究中起到越来越重要的作用。Atomic force microscopy （AFM） has been applied in many biological investigations in recent years, and this review focuses on the application of AFM in DNA-protein interactions. AFM images of static DNA- protein complexes, in air and in liquid, can be used to obtain quantitative and qualitative information on the structure of different complexes. And dynamic AFM images of DNA-protein complexation in real time under liquid conditions will help to understand biological processes and mechanisms at single molecule level. In addition, the measurement of intermolecular forces between biomolecules also provides new opportunities for studying mechanical properties of biomolecules and the interactions in their native environment. AFM has revealed many mechanisms of gene regulation, and will play a more and more important role in life science research.

关 键 词：原子力显微镜 DNA 蛋白质 相互作用 动力学 

分 类 号：Q343[生物学—遗传学]