流式细胞术检测巨噬细胞吞噬功能的方法建立  

Establish a New Method of Detecting Phagocytosis of Macrophage by Flow Cytometry

作　　者：陈连山[1] 张洋[1] 苏玉虹[1] 

机构地区：[1]辽宁医学院,锦州121001

出　　处：《数理医药学杂志》2007年第5期622-624,共3页Journal of Mathematical Medicine

摘　　要：目的:建立用流式细胞术检测小鼠腹腔诱导的巨噬细胞吞噬功能的方法。方法:用贴壁法筛选有活性的巨噬细胞,再与用碳酸盐缓冲液制备的荧光素(FITC)标记的大肠埃希菌混合,37℃孵育,每10min取少量固定后加EB染巨噬细胞核,用流式细胞仪检测,计算巨噬细胞对大肠埃希菌的吞噬率。结果:FITC能够有效标记大肠埃希菌;小鼠腹腔诱导的巨噬细胞能够吞噬大肠埃希菌,并且在30min时达到最大峰值。结论:用流式细胞术检测小鼠巨噬细胞吞噬FITC标记大肠埃希菌是测定巨噬细胞吞噬功能简便、快速和重复性好的定量方法。Objective： To use a new method of detecting phagocytosis of macrophage induced from the Belly of mouse by flow cytometry. Methods： Firstly, the living macrophages were collect by cultivating and adherencing, and bacterium coli（E, coli） was labeled FITC. Secondly, E. coli was mixed with macrophages, every 10 minute to take out of small amounts of mixed liquor,and add a little of EB dye. Finally, the fluorescent intensity was measured by flow cytometry. Results： The E. coli was labeled well by FITC in carbonate buffer. Macrophage can phagocytize E. coli and the efficient rat of phagocytosis was highest about at 30 minute. Conclusion： The method that detecting phagocytosis of macrophage phagocytizs E. coli is convenient. fast.good repeatability.

关 键 词：巨噬细胞 吞噬作用 大肠埃希菌 流式细胞仪 

分 类 号：Q2-33[生物学—细胞生物学]