金富猕猴桃离体培养与植株再生的优化研究  

Studies on Optimization of the Conditions for in vitro Culture and Plantlet Regeneration of Actinidia chinensis cv. JinFu

作　　者：刘长春[1] 陈泽雄[1] 龚雪芹[1] 刘奕清[1] 

机构地区：[1]重庆文理学院花卉研究所生命科学系重庆高校园林花卉工程研究中心,重庆永川402168

出　　处：《西南师范大学学报（自然科学版）》2007年第5期124-128,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：重庆市教委重大平台建设项目(GCZX0713);重庆文理学院重点科研项目

摘　　要：以"金富"猕猴桃的单芽茎段为外植体进行离体培养和植株再生的优化研究.结果表明,以MS+6-BA1.0 mg/L+NAA 0.1 mg/L作增殖继代培养基为宜,以1/2MS+NAA 0.3 mg/L作生根培养基为宜.适当浓度的6-BA对猕猴桃的不定芽增殖有明显的促进作用,而对茎伸长却有抑制作用,6-BA和NAA配合使用可促进芽苗主茎伸长,对改善猕猴桃植株组培苗质量有较好的作用.炼苗移栽成活率达到94%以上.Stem segments with a single bud of Actinidia chinensis cv. JinFu were used as explants in culture in vitro and for rapid propagation. The results indicated that MS ＋ 6-BA 1.0 mg/L＋NAA 0. 1 mg/ L was a suitable medium for shoot multiplication, and the best medium for rooting was 1/2MS ＋ INAA 0. 3 mg/L. 6-BA used in proper concentrations promoted shoot multiplication but inhibited stem elongation. Combined application of 6-BA and NAA promoted the elongation of the stem segments. The survival rate of hardened regenerated plantlets was as high as 94% after transplanting.

关 键 词：猕猴桃 组织培养 植株再生 优化研究 

分 类 号：Q949.758.2[生物学—植物学]