百日草根尖和花药愈伤组织染色体制片技术  

Chromosome technique of root tips and anther callus in Zinnia elegans

作　　者：叶要妹[1] 陈天花[1] 齐迎春[1] 杨涛[1] 

机构地区：[1]华中农业大学园艺林学学院教育部园艺植物生物学重点实验室,武汉430070

出　　处：《广西植物》2007年第5期673-675,740,共4页Guihaia

基　　金：国家"948"项目(2003-Z36)~~

摘　　要：以种子萌发根尖和花药愈伤组织为材料,研究了取样时间、预处理方法对百日草染色体制片的影响。结果表明:根尖上午8:00～9:00,花药愈伤继代3～5d上午9:00～10:00为最佳取样预处理时间;采用三种药剂预处理活体根尖,以4℃下饱和对二氯苯溶液或0.002mol/L的8-羟基喹啉液预处理8h效果最佳,花药愈伤则以饱和对二氯苯溶液预处理6h效果最佳。本实验的预处理温度是固定的,可克服预处理随季节和时间温度的变化而带来的不稳定性,且百日草花药愈伤染色体观察为首次报道。The roots of germinating seeds and anther callus were used to study the effects of the sampling time, the pretreatment on the slide-preparation of the chromosomes in Zinnia elegans Jacq. The results indicated that the optional root-sampling time was about 8：00-9：00 am,anther callus about 9：00-10：00 am for 3-5 d. Of the three chemicals used to pre-treating the intravital root tips, P-Dichlorobenzene solution or 0. 002 mol/L 8-Hydroxyquinoline for 8 hours at 4 ℃ had the better effect,and anther callus P-Dichlorobenzene solution for 6 hours at 4 ℃. These methods indicated that the number of metaphase chromosomes appeared more and chromosome spread well. The temperature of pretreatment in the study was fixed,overcame instability of effect with variance of season and time. Observation of chromosome of anther callus in Zinnia elegans Jacq. was first reported.

关 键 词：百日草 根尖 花药愈伤组织 预处理 染色体制片 

分 类 号：Q94-336[生物学—植物学] S681.9[农业科学—观赏园艺]