大鼠急性肺栓塞后hnRNP-E1的表达及胶原代谢的变化  

Up-regulated hnRNP-E1 enhances collagen metabolism in lung tissues after acute pulmonary embolism

作　　者：李圣青[1] 欧阳海峰[1] 刘阿茹[1] 简文[1] 遆新宇[1] 任新玲[1] 赵峰[1] 

机构地区：[1]第四军医大学西京医院呼吸内科,西安710033

出　　处：《第三军医大学学报》2007年第20期1968-1971,共4页Journal of Third Military Medical University

摘　　要：目的研究大鼠急性肺栓塞模型肺组织中异源性核糖核蛋白E1(heterogeneous nuclear ribonucleoprote E1,hnRNP-E1)的表达变化及其对胶原代谢的影响。方法建立大鼠急性肺栓塞模型,分别在急性肺栓塞后1、2、7和21d开胸取出肺组织,然后提取肺组织的总RNA和总蛋白,以正常大鼠为对照组,采取半定量RT-PCR的方法研究hnRNP-E1在mRNA水平表达的变化;采用Western blot方法进一步验证hnRNP-E1在蛋白水平表达的变化。采用Northern blot法分析大鼠肺组织中Collagen α1(Ⅰ)(COL1A1)和Collagen α1(Ⅲ)(COL3A1)在肺栓塞后mRNA水平的表达变化;采用羟脯氨酸(HYP)和Masson染色观察急性肺栓塞3周后肺组织内的胶原沉积状况。结果在大鼠急性肺栓塞后的不同时间点,hnRNP-E1的mRNA水平和蛋白水平的表达均有显著升高。大鼠急性肺栓塞后肺组织中COL1A1的mRNA表达水平有明显升高,但是COL3A1的mRNA表达水平无显著变化。HYP分析和Masson染色均显示急性肺栓塞3周后肺组织内的胶原沉积明显增加。结论大鼠急性肺栓塞后肺组织内hnRNP-E1的表达升高,可能促进了COL1A1的蛋白表达和胶原在肺部的沉积。Objective To study the expression changes of heterogeneous nuclear ribonucleoprotein E1 （hnRNP-E1） in the lung tissues after acute pulmonary embolism （PE） in rats and its effects on the metabolism of collagen. Methods The Wistar rat model of acute PE was established by injecting 3 -4 emboli into the left jugular vein. The lung tissue samples were collected at day 1,2, 7 and 21 after acute PE, then the total RNA and total proteins were obtained. The normal rats were used as control. The mRNA level changes of hnRNP-E1 were identified by semi-quantitative RT-PCR, and its protein level changes were validated by Western blot. The relative amounts of steady state Collagen α1（ Ⅰ ） （ COL1A1 ） and Collagen α1 （ m ） （ COL3A1 ） mRNA in the lung tissues after acute PE were analyzed by Northern blot. Hydroxyproline （HYP） determination and Masson dyeing were employed to observe the deposition of collagen in the lung tissues 3 weeks after acute PE. Results At different time points, the mRNA levels and the protein levels of hnRNP-E1 increased gradually in the rat lung tissues after acute PE. The mRNA levels of COL1 A1 increased steadily after acute PE, but those of COL3A1 had no changes. The deposition of collagen in the lung tissues increased obviously 3 weeks after acute PE. Conclusion The expression of hnRNP-E1 is up-regulated after acute PE. It might facilitate the deposition of collagen in the lung tissues.

关 键 词：肺栓塞 急性 蛋白印迹 半定量逆转录-多聚酶链式反应 hnRNP—E1 

分 类 号：R332[医药卫生—人体生理学] R446[医药卫生—基础医学]