妊娠相关血浆蛋白-A与促性腺激素在促超排卵中相关性研究  

Study on the role of pregnancy-associated plasma protein-A and gonadotropin in the development of ovarian follicle during superovulation stimulation

作　　者：刘蓓[1] 章晓梅[2] 李永刚[2] 王卫疆[1] 

机构地区：[1]中国人民解放军昆明总医院妇产科,昆明650032 [2]云南省第一人民医院生殖遗传科,昆明650032

出　　处：《生殖医学杂志》2007年第5期342-346,共5页Journal of Reproductive Medicine

摘　　要：目的探讨超排卵周期中妊娠相关血浆蛋白-A(PAPP-A)与卵泡刺激素(FSH)和黄体生成素(LH)对卵泡发育是否有协同效应。方法对27例因输卵管因素行体外受精-胚胎移植(IVF-ET)的患者,给予相同的超促排卵方案,采用逆转录-聚合酶联反应(RT-PCR)和酶联免疫吸附试验(ELISA)法检测患者取卵时卵泡液中PAPP-A水平;化学发光免疫法检测用药前(月经第2天)、用药后(hCG日)血清FSH和LH水平。按取卵时卵泡平均直径≥14 mm数目将卵巢反应分为3型:低、中和高反应型。结果卵泡液PAPP-A水平高、中反应型显著高于低反应型,差异有显著性(P<0.01,P<0.05);血清FSH、LH水平高、中反应型均显著高于低反应型,差异有显著性(P<0.05);PAPP-A、FSH、LH三者呈正相关关系。结论超促排卵周期中,PAPP-A和促性腺激素在促进卵泡发育中可能有协同作用。Objective： To explore the roles of pregnancy-associated plasma protein-A （PAPP-A） and gonadotropin in the development of ovarian follicle during superovulation cycles. Methods： 27 patients who underwent IVF-ET because of tubal infertility were treated with the same superovulation regime. These patients were divided into three groups （low, moderate and high reactive group） according to the number of ovarian follicles with diameter ≥14 mm. PAPP-A level of the follicular fluid was measured with RT-PCR and ELISA on the day of oocyte pick-up （Opu）. Serum FSH and LH levels were analyzed by radioimmunoassay before and after superovulation. Results： As compared to that in low reactive group, PAPP-A level of the follicular fluid in moderate and high reactive groups was markedly elevated （P〈0. 01 ,P〈0. 05, respectively）. Similarly, serum FSH and LH levels in moderate and high reactive groups were also higher than those in low reactive group （P〈0.05）. Positive correlation was found among follicular fluid PAPP-A, serum FSH and LH. Conclusion： PAPP-A and gonadotropin may coordinate to promote the development of follicles during gonadotropin stimulation in IVF-ET program.

关 键 词：妊娠相关血浆蛋白-A 促性腺激素 超排卵 卵泡 卵泡液 

分 类 号：R711.6[医药卫生—妇产科学]