正交实验法优选消喘贴膏提取工艺  

Study on Optimum Preparation Process of Xiaochuan Tiegao by Orthogonal Design

作　　者：张萱[1] 刘起华[1] 文谨[1] 

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《中国中医药信息杂志》2007年第10期48-49,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：中国中医科学院广安门医院资助课题(2004s77)

摘　　要：目的优选消喘贴膏的最佳提取工艺。方法采用高效液相色谱法,测定方中白芷的主要成分欧前胡素含量,用L9(34)正交设计法筛选乙醇提取的最佳条件。结果消喘贴膏的最佳提取工艺为:乙醇浓度90%,溶剂用量为2倍药材量,回流时间1h,回流次数2次。结论该提取工艺稳定,提取率高。Objective To optimize the preparation process of Xiaochuan Tiegao. Methods The effective compound imporatorin of Dahurian Angelica Root was determined by HPLC. The best condition of preparation process for the ethanol-extraction method was investigated by L9（3^4） orthogonal design. Results The optimum technical condition： adding 2 times ethanol, cooking 1 hour and 2 times with 90% ethanol. Conclusion The method is reliable, accurate and easy to operate.

关 键 词：消喘贴膏 欧前胡素 高效液相色谱法 正交设计 

分 类 号：R283.5[医药卫生—中药学]