聚马来松香乙二醇酯Cu(II)配合物固定化漆树漆酶的研究  

IMMOBILIZATION OF RHUS LACCASE ON THE CUPRUM-COMPLEXED MALEATED ROSIN-ETHYLENE GLYCOL ESTER POLYMER

作　　者：蓝虹云[1,2] 刘祖广[1,2] 雷福厚 黄道战[1,2] 韩金华[1,2] 

机构地区：[1]广西民族大学化学与生态工程学院,南宁530006 [2]广西林产化学品开发与应用重点实验室,南宁530006

出　　处：《离子交换与吸附》2007年第5期415-420,共6页Ion Exchange and Adsorption

基　　金：广西科学研究与技术开发基金(桂科攻0630004-1);广西高校松香新产品开发人才小高地资助项目

摘　　要：制备了聚马来松香乙二醇酯（MEEP）Cu^2＋、Ni^2＋、Ca^2＋和Mg^2＋金属离子配合物，并分别以4种配合物为载体固定化漆树漆酶，初步探讨了反应时间对酶固定化的影响，考察了固定化酶的性质。实验结果表明，在反应温度为25℃条件下，漆树漆酶的最佳固定化时间为16h；MEEPCu^2＋配合物固定化酶的固定化结果较好，重复使用6次后，酶相对保留活力为55．0％；该固定化酶的最适作用温度为40℃，pH值范围为5．89—9．23，固定化漆树漆酶具有比游离酶更好的热稳定性和更广泛的pH值适用范围。A series complexes of maleated rosin-ethylene glycol ester polymer （MEEP） were prepared with metal ions Cu^2＋, Ni^2＋, Ca^2＋ and Mg^2＋. These complexed polymer were used as carders for immobilizing rhus laccase. The reaction time and the properties of the immobilized rhus laccase were studied. The results showed that the favorable reaction time was 16h at 25℃. The immobilized rhus laccase on cuprum-complexed MEEP （MEEPCu^2＋） had better reusability, it still kept 55.0% of the activity after being used 6 times. The optimum operating temperature was 40℃ for immobilized rhus laeease which was 10℃ higher than that of free rhus laeease. Suitable pH value range was 5.89-9.23. The immobilized rhus laccase had better thermal stability and more extensive optimum pH value than free rhus laccase.

关 键 词：聚马来松香乙二醇酯 金属离子 配合物 漆树漆酶 固定化 

分 类 号：TQ351.47[化学工程] O636[理学—高分子化学]