RP-HPLC测定茵栀黄口服液中黄芩苷含量  

Determination of Baicalin inYinzhihuang Oral Solution by RP-HPLC

作　　者：白保国[1] 

机构地区：[1]济宁市第二人民医院,山东济宁272149

出　　处：《中国现代中药》2007年第10期21-23,共3页Modern Chinese Medicine

摘　　要：目的:应用RP-HPLC测定茵栀黄口服液中黄芩苷的含量。方法:采用BDSHYPERSILC18色谱柱(250mm×4.6mm,5μm),流动相:甲醇-0.2%磷酸(47∶53),流速:1.0mL·min-1,柱温:35℃,检测波长:277nm。结果:在上述色谱条件下,黄芩苷对照品进样量在5.88～235.2μg·mL-1与其峰面积呈良好的线性关系,r=0.9995。平均回收率为99.6%,RSD=1.07%。结论:此方法简便、准确、灵敏,可用于该制剂的质量控制。Objective： To determine the content baicalin inYinzhihuang oral solution by RP - HPLC. Methods： The BDS HYPERSIL- C18 column （250mm × 4.6mm, 5μm） was used. The mobile phase was methanol- 0.2% phosphoric acid solution （47：53 ） The detection wavelength was 277nm, the flow rate at 1.0mL. min^- 1 and the column temperature at 35 ℃. Results： Baicalin showed a good linear relationship within a range of 5.88 -235.2 μg.mL^-1 （r = 0. 999 5） ; The average recovery was 99.6% （RSD = 1.07% ） . Conclusion： The method is simple, accurate and sensitive. It can be used for quality control on the preparation.

关 键 词：RP-HPLC 黄芩苷 茵栀黄口服液 

分 类 号：R927.2[医药卫生—药学]