金龟子绿僵菌对多菌灵的室内抗药突变体的诱导及其抗药性  

Induction of Laboratory Mutants of Metarhizium anisopliae and Its Resistance to Carbendazim

作　　者：许天委[1] 詹儒林[1] 郭立佳[1] 宋妍[1] 张世清[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所

出　　处：《热带作物学报》2007年第2期97-101,共5页Chinese Journal of Tropical Crops

基　　金：国家科技部"科技基础性工作和社会公益研究专项"(编号:2004DIB3J073);科技部"国家科技基础条件平台工作"(编号:2004DKA30560)等资助

摘　　要：紫外线(UV)照射金龟子绿僵菌(Metarhizium anisopliae)MA4菌株后,从中分离筛选到高抗多菌灵(MBC)的突变菌株。对其中部分突变菌株的抗药稳定性分析,突变菌株MA4-1在无多菌灵培养基中继代培养10代后,仍然能在600μg/mL的多菌灵浓度下生长,而菌株MA4只能在0.5μg/mL多菌灵浓度下生长。结果表明:突变菌株MA4-1抗多菌灵浓度提高了1200倍,且抗药性稳定。方差分析结果表明:菌株MA4与突变菌株MA4-1,MA4-2,MA4-3的几丁质酶活力不存在显著差异。Carbendazim-resistant mutants of Metarhizium anisopliae strain（MA4） were generated in the laboratory by ultra-violet （UV）. The resistance of the generated mutants to carbendazim was investigated. The mutants grew well by asexual reproduction in PDA and also grew at the concentration of 600 μg/mL of MBC even after 10 times of subculture, but the sensitive strain（MA4） only grew at the concentration of 0.5 μg/mL MBC. The results showed that the mutants increased its resistance to carbendazim by 1 200 times and was very stable in resistance. At the same time, the ANOVA demonstrated no significant difference in chitinase activity between the strain MA4 and the mutants MA4-1, MA4-2 and MA4-3.

关 键 词：金龟子绿僵菌 UV 多菌灵 几丁质酶 

分 类 号：S433.5[农业科学—农业昆虫与害虫防治] S476.12[农业科学—植物保护]