硬皮病小鼠CTGF、I型胶原含量及其相关性分析  

Effects of connective tissue growth factor and collagen type I on scleroderma

作　　者：闫小宁[1] 冯捷[1] 张彩晴[1] 李文彬[1] 崔荣[1] 

机构地区：[1]西安交通大学第二附属医院,西安710004

出　　处：《陕西医学杂志》2007年第7期783-785,共3页Shaanxi Medical Journal

摘　　要：目的:探讨结缔组织生长因子(CTGF)、I型胶原蛋白(COL-I)在硬皮病发病中的作用及其二者的相关性。方法:对12只小鼠采用博来霉素皮下注射形成硬皮病模型,免疫组化Elivision法检测硬皮病模型的皮损及肺组织中CTGF蛋白及COL-I含量,以12例正常小鼠皮肤、肺组织作为对照,并对CTGF及COL-I的含量及肺组织形态定量分析比较和相关分析。结果:CTGF及COL-I在硬皮病小鼠皮损、肺组织的含量均高于对照组;CTGF含量与COL-I含量呈正相关。肺组织形态定量分析模型组较对照组纤维化加深。结论:CTGF及COL-I在小鼠皮肤硬化中起重要作用,可能与硬皮病的病理纤维化的形成有关。Objective： To investigate the effects of connect tissue growth factor （CTGF） and collagen type I （COL-I ） on the pathogenesis of scleroderma and explore the relationship between the content of COL-I and CTGF. Methods： 12 scleroderma mice models were established by the injection of Bleomycin. The CTGF and COL-I level were detected by immunohistochemical method. The relationships were analyzed between CTGF and COL-I level. As control group, 12 healthy mice were also detected. Results： The level of CTGF and COL-I in sclerotic models were higher than in normal controls . It was found that there was a correlation between the level of CTGF and COL-I. Conclusion： CTGF and COL-I play an important role in the hardening process of the skin lesions of the mice model of sclerotic skin, which may be involved in the pathogenesis of scleroderma.

关 键 词：硬皮病 局限性/病理生理学 生长物质/分析 胶原 型/分析 小鼠 

分 类 号：R331[医药卫生—人体生理学]