甲状腺肿瘤原位细胞凋亡检测与PTEN表达研究  

Significance of cell apoptosis index and PTEN in thyroid tumor tissue

作　　者：钟宇华[1] 梁华晟[1] 梁秀就[2] 周少碧[1] 黄宇[3] 詹松[1] 

机构地区：[1]广西医科大学第九附属医院,北海536000 [2]广西医科大学第一附属医院病理科 [3]广西壮族自治区人民医院肝胆外科

出　　处：《陕西医学杂志》2007年第7期873-874,883,共3页Shaanxi Medical Journal

基　　金：广西科技厅自然科学基金资助(桂科自NO.0339080)

摘　　要：目的:研究原位细胞凋亡指数(AI)及抑癌蛋白(PTEN)在原发性甲状腺肿瘤中表达及其与病变的关系。方法:对87例原发性甲状腺肿瘤分别应用原位细胞凋亡检测(TuNEL)试剂盒测定AI及应用免疫组化方法检测PTEN表达情况。结果:AI及PTEN蛋白在各甲状腺肿瘤组织均存在表达,AI在腺癌表达高于腺瘤,PTEN在乳头状癌及腺瘤表达显著高于滤泡性癌及未分化癌;AI及PTEN有正相关性。结论:AI及PTEN的表达与甲状腺肿瘤显著相关,检测AI及PTEN水平对于鉴别原发性甲状腺滤泡癌具有重要参考意义。Objective： To study the relationship between Apoptosis Index（AI）,PTEN and thyroid tumor tissue by combining assays of AI and PTEN in thyroid tumor tissue. Methods： AI was evaluated by TUMEL method and the expression of PTEN were dectected by immunohistochemistry in 78 cases. Results： The expression of AI in thyroid carcinoma increased. The expression of PTEN in thyroid adenoma and papillary cancer are higher than thyroid follicular cancer and undifferentiated cancer.There has positive relationship between AI and PTEN. Conclusion： It is suggested that combined detections of AI and PTEN may have important reference significance on the differentiation between thyroid papillary tumor tissue.

关 键 词：甲状腺肿瘤/病理学 @PTEN基因 细胞凋亡/免疫学 免疫组织化学 

分 类 号：R653[医药卫生—外科学]