降钙素原和C-反应蛋白预测和鉴别大鼠急性胰腺炎并感染的研究  

Procalcitonin and C-reactive protein in the prediction and differentiation of acute pancreatitis with infection in rats

作　　者：梁浩晖[1] 詹勇强[1] 王成友[1] 张敏杰[1] 倪勇[1] 程书榜[1] 

机构地区：[1]广东省深圳市第二人民医院肝胆外科,广东深圳518035

出　　处：《中国普通外科杂志》2007年第10期979-981,共3页China Journal of General Surgery

基　　金：广东省深圳市科技计划项目(200405031)

摘　　要：目的探讨血浆降钙素原(PCT)及C-反应蛋白(CRP)对早期预测急性胰腺炎并感染的价值。方法80只SD大鼠随机分为(1)急性胰腺炎并感染组(I组,n=20);(2)急性胰腺炎对照组(C组,n=40),根据成模后腹腔有感染再分为C1和C2组;(3)假手术对照组(S组,n=20),采集成模前(0h)及成模后12,24h及48h时点血液和48h时点胰腺及肝脏组织,用ELISA法检测血浆CRP的浓度,WesternBlot检测血浆和肝脏PCT水平结果⑴B组大鼠腹腔均发生感染,C1组中16例腹腔发生感染,C2组20例未发生感染。⑵B组和C1组血浆CRP水平呈持续上升趋势,在成模后48h均显著高于C2组。⑶B组和C1组血浆及肝脏组织内于成模后48h均能检到较高水平的PCT,且明显高于C2组及S组。结论PCT对急性胰腺炎早期感染具有一定的预测作用;联合血浆CRP检测有助于鉴别胰腺炎是否合并感染。肝脏组织可能是PCT的一个重要合成部位。Objective To study the early prediction of infection in acute pancreatitis in rats by plasma procalcitonin （ PCT ） and c- reactive （ CRP ） detection. Methods Eighty SD rats were randomly assigned into acute infected pancreatitis group （I, n = 20 ）, pancreatitis control group （C, n = 40 ） and sham-operated group （S, n = 20）. Blood samples were collected pre- （Oh） and post-operatively （12h, 24h and 48h）. Plasma CRP was analyzed by ELISA. Plasma and liver PCT was detected by Western blot. Results （ 1 ） . Ascitic infection occurred in all the group B rats and 16 of 40 rats of group C （ analyzed as group C1 ） , and did not occur in the other 20 of 40 rats of group C （analyzed as group C2） and group S. （2）. The plasma CRP concentrations elevated gradually after the model setup in group B and C1, which were significantly higher at 48h than those in group C2 and group S. （3）. PCT was detected in high levels in plasma and liver tissues in group B and C1 at 48h post-operatively, and they were sighificantly higher than those in group C2 and group S. Conclusions PCT can predict early infection of acute pancreatitis, and detection of PCT combined with plasma CRP may help in the differentiation of acute infected pancreatitis. The liver may be an important organ for synthesis of PCT.

关 键 词：胰腺炎/病理学 感染/并发症 降钙素原 C-反应蛋白 疾病模型 动物 大鼠 

分 类 号：R657.51[医药卫生—外科学]