血清精子蛋白17检测的双抗夹心ELISA法及在卵巢癌诊断中的应用  

Development of double antibodies sandwich ELISA method for detecting Sp17 in serum and its primary clinical application

作　　者：缪家文 李芳秋[2] 杨爱龙[2] 

机构地区：[1]镇江市第三人民医院中心实验室,江苏镇江212019 [2]南京军区南京总医院全军医学检验中心,江苏南京210002

出　　处：《江苏大学学报（医学版）》2007年第5期445-447,450,共4页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省"六大人才高峰"基金资助项目(2005A2)

摘　　要：目的:建立双抗夹心ELISA法检测血清中精子蛋白17(sperm protein 17,Sp17)水平。方法:以重组Sp17蛋白为免疫原制备兔抗Sp17抗体,建立Sp17双抗夹心ELISA检测方法,鉴定其最小检出限、精确度、回收率,并检测42例正常人及28例卵巢癌患者血清Sp17水平。结果:该双抗夹心ELISA法敏感度高、特异性强、重复性好。卵巢癌患者与正常人血清Sp17含量有显著差别(P<0.01),正常人血清中存在少量Sp17蛋白,而卵巢癌患者血清中Sp17含量明显升高者占39.3%。结论:卵巢癌患者血清中Sp17含量明显升高。测定血清Sp17水平的ELISA方法学确立为阐明Sp17在卵巢癌患者中的免疫生物学意义提供了实验手段。Objective： To develop double antibodies sandwich ELISA for detecting sperm protein 17 （Sp17） in serum and investigate clinical significance of serum Sp17 for ovarian carcinoma. Methods： Prepared rabbit antiserum of Sp17 by using a purified recombinant Sp17 protein as antigen, double antibodies sandwich ELISA for serum Sp17 was established and serum Sp17 was detected in ovarian carcinoma and normal control. Results： Sp17 polyclonal antibody was preparationed and it＇s specificity with Sp17 in human testis was confirmed by immunohistochemistry. The double antibodies sandwich ELISA for serum Sp17 has been high sensitive, repeated and specific. There are obvious differences comparing ovarian carcinoma group with normal control group （P 〈 0.05 ）. The serum Sp17 level in 39.3% ovarian carcinoma was significantly high. Conclusion： The serum Sp17 level in ovarian carcinoma was significantly higher than in healthy persons. The establishment of ELISA method for serum Sp17 provides the experimental means to illustrate the imunobiological significance of Sp17 in ovarian carcinoma.

关 键 词：精子蛋白17(Sp17) 卵巢癌 ELISA 

分 类 号：R446.61[医药卫生—诊断学] R737.31[医药卫生—临床医学]