Humidity Effects on Imaging and Nanomanipulation of Individual DNA Molecules on HOPG Surface  

作　　者：周星飞 张益 王化斌 牛冬校 王培卿 胡钧 

机构地区：[1]Department of Physics, College of Sciences, Ningbo University, Ningbo 315211 [2]Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800 [3]Bio-X Life Science Research Center, Shanghai Jiao Tong University, Shanghai 200240

出　　处：《Chinese Physics Letters》2007年第9期2692-2695,共4页中国物理快报（英文版）

基　　金：Supported by the National Natural Science Foundation, Chinese Academy of Sciences and Zhejiang Natural Science Foundation.

摘　　要：Single DNA molecules are aligned on highly ordered pyrolytic graphite （HOPG） surface in ambient air. It is shown that environmental humidity has a remarkable influence on the measured height of DNA by atomic force microscopy （AFM）, probably due to the conformation transition of DNA. We also demonstrate that DNA molecules deposited on HOPG surface can be ＇pushed＇ much more easily by AFM tip at high humidity than at 10W one.Single DNA molecules are aligned on highly ordered pyrolytic graphite （HOPG） surface in ambient air. It is shown that environmental humidity has a remarkable influence on the measured height of DNA by atomic force microscopy （AFM）, probably due to the conformation transition of DNA. We also demonstrate that DNA molecules deposited on HOPG surface can be ＇pushed＇ much more easily by AFM tip at high humidity than at 10W one.

关 键 词：coated conductor buffer layer self-epitaxy CEO2 

分 类 号：O56[理学—原子与分子物理]