慢性乙型肝炎、肝硬化患者外周血LAK细胞活性和sIL-2R测定的意义  

SIGNIFICANCE OF MEASURING THE ACTIVITY OF LAK CELLS DERIVED FROM PERIPHERAL BLOOD CELLS AND SERUM LEVEL OF sIL 2R IN PATIENTS WITH CHRONIC HE PATITIS B AND LIVER CIRRHOSIS

作　　者：覃金爱[1,2] 周桂英[1,2] 刘革 罗光汉 吴继周 

机构地区：[1]广西民族医院 [2]广西医科大学第一附属医院

出　　处：《免疫学杂志》1997年第2期103-104,共2页Immunological Journal

摘　　要：采用３Ｈ－ＴｄＲ释放法测定５１例慢性肝病患者（ＣＰＨ１０例、ＣＡＨ２３例、ＬＣ１８例）外周血ＬＡＫ细胞活性，并用酶联法测定患者血清中ｓＩＬ－２Ｒ含量；与２９例正常对照组比较，发现肝病患者ＬＡＫ活性降低，ＨＢＶＤＮＡ阳性组ＬＡＫ活性较阴性组低（Ｐ＜０．０５），ｓＩＬ－２Ｒ增高，且慢性肝病组ＬＡＫ活性与ｓＩＬ－２Ｒ水平呈负相关，说明ＬＡＫ活性与机体免疫功能状态有关，ＨＢＶ的复制和高浓度的ｓＩＬ－２Ｒ可影响ＬＡＫ活性。The activity of lymphokine activated killer cells derived from peripheral blood mononuclear cells of 29 normal controls,51 patients with chronic liver diseases (10 chronic persistent hepatitis B,23 chronic active hepatitis B and 18 liver cirrhosis) were measured by 3H TdR release assay Soluble interleukin 2 receptor in sera of these patients and healthy individuals were quantified with enzyme linked immunosorbent assay The results that LAK activity of patients with chronic liver diseases were decreased when compared to that of controls In chronic active hepatitis group,LAK activity of patients with positive HBVDNA marker was lower than that of patients with negative HBVDNA marker ( P < 0 05 ) Patients with chronic hepatitis B and liver cirrhosis had higher serum sIL 2R concentrations than controls We also found that LAK activity was negative correlated remarkably with serum level of sIL 2R in patients with chronic hepatitis B These findings suggested that LAK activity was related with immune state of organism The replication of HBV and high serum concentration of sIL 2R can interfere in LAK activity

关 键 词：乙型肝炎 肝硬化 LAK细胞 SIL-2R 外周血 

分 类 号：R512.620.3[医药卫生—内科学] R575.203[医药卫生—临床医学]