甲烷利用菌催化烯烃环氧化的底物选择性,细胞失活原因及产物对映体组成  

Substrate Selectivity, Cell Deactivation, Enantiomeric Composition in Epoxidation of Alkenes Catalyzed by Methane - Utilizing Bacteria

作　　者：夏仕文[1] 李树本[1] 尉迟力[1] 阮宗琴[1] 

机构地区：[1]中国科学院兰州化学物理研究所

出　　处：《化学学报》1997年第1期76-82,共7页Acta Chimica Sinica

基　　金：国家自然科学基金资助课题

摘　　要：研究了甲烷利用菌Methylomonas sp.GYJ3,Methylomonas sp.S,Methylomonaa sp.Z201,Methylococcus capsulatus IMV3021,Methylosinus trichosporium IMV3011休止细胞催化烯烃环氧化的底物选择性,细胞失活原因以及产物对映体组成.发现不同菌株和底物的环氧化活性不同.甲烷利用菌只能催化短链烯烃环氧化,环烯烃和芳香烯烃无反应.对烯丙基型底物而言,取代基大小和极性影响环氧化活性 丙烯环氧化活性最高,烯丙醇不能环氧化.细胞失活的主要原因是环氧化产物的细胞毒性和反应体系中辅酶NADH损耗.手性气相色谱揭示甲烷利用菌催化烯烃环氧化形成外消旋产物.Substrate selectivity,reasons for cell deactivation,enantiomeric composition of products in the epoxidation of alkenes catalyzed by methane - utilizing bacteria resting cell ( Methylomonas sp. GYJ3 , Methylomonas sp. S, Methylomonas sp. Z201, Methylosinus trichosporium IMV3011, Methylococcus capsulatus IMV 3021)were studied. There were different epoxidizing activities for various bacterial strains and various substrates. Methane - utilizing bacteria were unable to epoxidize cyclohexene and srgrene,but short - chain alkenes. Activity of epoxidation was related to steric effect for allyl substrates XCH2CH = CH2(X= H,Cl,Br,OH). The main reasons for cell deactivation were cell - toxicity of products and lost of coenzyme NADH. Chiral capillary gas chromatography revealed that epoxidizing products of alkenes were racemic epoxides.

关 键 词：烯烃 环氧化 底物选择性 甲烷利用菌 催化 

分 类 号：O623.12[理学—有机化学] O643.36[理学—化学]