CaM BP-10调节生长素应答反应机理初探  

PRELIMINARY INVESTIGATION ON REGULATORY MECHANISM OF CaM BP-10 ON CELL RESPONSES TO AUXIN

作　　者：凌启阆[1] 刘华[1] 向左云[1] 尚克进 

机构地区：[1]南开大学生物化学及分子生物学系,天津300071

出　　处：《南开大学学报（自然科学版）》1997年第2期101-103,共3页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金

摘　　要：前期研究发现CaMBP－10参与植物细胞对生长素应答反应的调节，但调节机理有待阐明．本文表明CaMBP－10是由于影响质膜H+－ATPase活性，进而影响了质子外泌和细胞伸长．并进一步证明，它对质膜H+－ATPase活性的调节是通过介导该酶磷酸化而实现的，最终调节了细胞对激素的应答．aM BP-10(BP-10) is a novel calmodulin (CaM) endogenous binding protein that wasobserved and isolated in plant by our research group. In preliminary studies, it was shownthat BP-10 was involved in the regulation on the responses of coleoptile to auxin. The present study shows that BP-10 inhibits cell responses to auxin through inhibiting the activityof plasma membrane H+-ATPase (PM H+-ATPase). In order to elucidate the regulatorymechanism of PM H+-ATPase activity by BP-10, the relationship between phosphorylation and activity of PM H+-ATPase has been investigated in vivo. The results prove thatBP-10 regulates the activity of PM H+-ATPase by mediating phosphorylation of the enzyme, as a result it affects cell responses to IAA.

关 键 词：钙调素结合蛋白 生长素 应答反应 植物细胞 

分 类 号：Q946.885.1[生物学—植物学]