含金属硫蛋白蛋奶粉对H-22小鼠肝癌的预防作用  

Preventive effects of egg-milk with MT on H-22 hepatocellular carcinoma in BALB/c mice

作　　者：赵红光[1] 徐松柏[2] 李艳博[1] 郭伟[1] 王志成[1] 史经宇 龚守良[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室 [2]吉林大学第一医院神经外科,吉林长春130021 [3]哈尔滨春源生物科技开发有限公司,黑龙江哈尔滨150036

出　　处：《吉林大学学报（医学版）》2007年第5期777-781,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30570546)

摘　　要：目的:观察含金属硫蛋白(MT)蛋奶粉对BALB/c小鼠H-22肝癌的预防作用。方法:雌性BALB/c纯系小鼠70只随机分为正常对照组(10只)、荷瘤鼠对照组(20只)、含MT蛋奶粉预防肿瘤低剂量及高剂量组(各20只)。将MT低和高剂量蛋奶粉给小鼠灌胃2周后,皮下接种H-22细胞,建立小鼠移植肿瘤模型,观察MT蛋奶粉对肿瘤出现时间及肿瘤大小和生长的影响;观察1个月后处死小鼠,MTT法检测脾脏淋巴细胞转化功能,流式细胞术检测脾脏CD4+、CD8+和CD25+T细胞百分率以及细胞周期进程和凋亡的变化,3H-TdR掺入法检测CTL和NK细胞杀伤活性。结果:与荷瘤对照组相比,含MT蛋奶粉组小鼠其肿瘤生长速率显著降低(P<0.05),淋巴细胞增殖率显著增加(P<0.01),脾脏CD4+和CD8+T细胞百分率及CD4+/CD8+比值显著升高(P<0.05或P<0.01),CD25+T细胞百分率和淋巴细胞凋亡百分率均显著降低(P<0.05或P<0.01),NK细胞毒性显著提高(P<0.05或P<0.01),CTL细胞毒性亦显著增高(P<0.01)。结论:含MT蛋奶粉可明显增强荷瘤小鼠的免疫功能,对小鼠移植性肿瘤的生长起到一定程度的抑制作用。Objective To explore the preventive effects of egg-milk with metallothionein （MT） on the H-22 hepatocellular carcinoma in BALB/c mice. Methods Seventy female BALB/c mice were divided into four groups at random： normal control （n= 10）, tumor control （n= 20）, low dose egg-milk with MT group （n= 20） and high dose egg-milk with MT group （n= 20）. The mice were injected with H-22 cells subcutaneously to build hepatocellular carcinoma model after they were administered with egg-milk with MT for two weeks. The developing time of tumor and tumor volume were detected. The mice were killed after one month and immune indices were measured with their spleens. The ability of lymphocyte proliferation was measured with MTT method, the percentages of CD4^＋ , CD8^＋ and CD25^＋ T cells and apoptotic percentage of lymphocytes were measured with flow cytometry, the killing activities of CTL and NK cells were detected with ^3H-TdR method. Results As compared with tumor group, the tumor developing speed in the mice administered with egg-milk with MT decreased significantly （P〈0. 05）, the lymphocyte proliferation rate increased significantly （P〈0.01）, the positive percentages of CD4^＋ , CD8^＋ T cells and the radio of CD4^＋/CD8^＋ T cells increased significantly （P〈0.05 or P〈0.01）, the positive percentage of CD25^＋ T cells and their apoptotic percentages decreased significantly （P〈0.05 or P〈0.01）, the toxicities of NK and CTL cells increased significantly （P〈0.05 or P〈0.01）. Conclusion Egg-milk with MT could enhance the immune function in the mice with hepatocyte tumor and inhibit the tumor developing speed obviously.

关 键 词：金属硫蛋白 H-22肝癌细胞 免疫功能 预防作用 

分 类 号：R459.3[医药卫生—治疗学] R735.7[医药卫生—临床医学]