西洋参叶20s-原人参二醇组皂苷对糖尿病肾病大鼠肾功能及肾脏结构的保护作用  

Protective effects of panax quinquefolium 20s-protopanaxdiolsaponins saponins on kidney function and structure in diabetic nephropathy rats

作　　者：臧晓峰[1] 谢湘林[1] 吴轶川[1] 刘凯[1] 李晔[1] 叶秋芳[1] 刘宏雁[1] 

机构地区：[1]吉林大学药学院药理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2007年第5期845-848,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅基金资助课题(990575-1)

摘　　要：目的:探讨西洋参叶20s-原人参二醇组皂苷(PQDS)对糖尿病肾病大鼠的影响。方法:88只Wistar大鼠腹腔注射链脲佐菌素(STZ)建立糖尿病肾病模型(DN),按空腹血糖值及体重将糖尿病肾病模型复制成功的大鼠按区组随机分组的方法分为模型组、PQDS50mg.kg-1组及100mg.kg-1组,每组15只,每天灌胃1次,连续灌胃35d,每10d测定1次血糖,35d后取血测定血糖、血清肌酐、尿素氮和肾重,通过光镜、电镜观察肾脏的结构。结果:与模型组比较,PQDS50mg.kg-1组糖尿病大鼠血糖、血肌酐及12h尿量差异无显著性(P>0.05),PQDS100mg.kg-1组糖尿病大鼠血糖降低、血肌酐及12h尿量减少(P<0.05),PQDS50和100mg.kg-1可降低肾脏细胞基质,减少系膜细胞增生,保护肾脏细胞的超微结构,改善大鼠糖尿病引起的肾脏病理损害。结论:PQDS可以降低糖尿病肾病大鼠血糖,改善肾脏功能,保护肾脏结构,对大鼠糖尿病肾病有保护作用。Objective To study the effects of panax quinquefolium 20s-protopanaxdiolsaponins （PQDS） on diabetic nephropathy （DN） rats. Methods DN model rats, made by streptozotocin （STZ）, were divided into model group, PQDS 50 and 100 mg · kg^-1 groups according to fasting blood glucose value and weight. The rats were given with PQDS by oral administration for 35 d. Every 10 d, the index of blood glucose was measured. On the last day, several indexes were measured including blood glucose, serum creatinine, UN, kidney weight, urine volume （within 12 h） and the structure of kidney was observed by light microscope and electron microscope. Results Compared with model group, the blood glucose and serum creatinine levels and 12 h urine volume were decreased in PQDS 100 mg · kg^-1 group （P〈0. 05）, but the indexes did not change in PQDS 50 mg· kg^-1 group （P〉0. 05）. PQDS （50 and 100 mg· kg^-1） decreased cell matrix and intercapillary cells, protected structure of kidney, improved renal pathological lesion in diabetic rats. Conclusion PQDS has therapeutic effects on DN rats through decreasing blood glucose, improving kidney function and preserving kidney structure.

关 键 词：西洋参叶20s-原人参二醇组皂苷 糖尿病肾病 链脲佐菌素 大鼠 Wistar 

分 类 号：R285.5[医药卫生—中药学] R587.2[医药卫生—中医学]