人参Rb组皂苷对大鼠实验性心肌缺血再灌注损伤的保护作用  

Protective effects of Ginsenoside-Rb on experimental myocardial ischemia-reperfusion injury in rats

作　　者：曲绍春[1] 睢诚[1] 于晓风[1] 王绚卉[1] 何小溪[1] 徐华丽[1] 

机构地区：[1]吉林大学药学院药理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2007年第5期849-852,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅新药基金项目资助课题(20000329)

摘　　要：目的:观察人参Rb组皂苷(G-Rb)对大鼠实验性心肌缺血再灌注损伤的保护作用。方法:在体大鼠结扎冠状动脉前降支30min,再灌注24h制备实验性心肌缺血再灌注损伤模型,G-Rb按25、50和100mg.kg-1.d-1给大鼠连续灌胃7d,假手术组及再灌模型组灌胃0.5%羧甲基纤维素钠,计算心肌梗塞范围(MIS),测定血清天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、心肌型肌酸激酶同功酶(CK-MB)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量,血浆前列环素(PGI2)及血栓素A2(TXA2)水平,测定血小板聚集活性。结果:与再灌模型组比较,G-Rb50、100mg.kg-1组大鼠的MIS缩小(P<0.05或P<0.01),血清AST、LDH、CK-MB活性及MDA含量降低(P<0.05或P<0.01),SOD及GSH-Px活性升高(P<0.05或P<0.01),血浆TXA2水平下降,PGI2水平及PGI2/TXA2比值增高(P<0.05或P<0.01),1、3和5min的血小板聚集率(PAR)及最大血小板聚集率(MPAR)降低(P<0.05或P<0.01)。结论:G-Rb对大鼠实验性心肌缺血再灌注损伤具有明显保护作用,可能与其增强抗氧化酶活性,减少自由基对心肌的氧化损伤,纠正PGI2/TXA2失衡以及抑制血小板聚集活性等机制有关。Objective To observe the protective effects of Ginsenoside-Rb （G-Rb） on experimental myocardial ischemia-reperfusion injury in rats. Methods The myocardial ischemia-reperfusion model was induced by 30 min left anterior descending coronary occulusion and 24 h reperfusion in openchest anesthetized rat. The changes of aspartate aminotransferase （AST）, lactate dehydrogenase （LDH）, MB isoenzyme of creatine kinase （CK-MB）, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） activities and malondialdehyde （MDA） content in serum, and prostacycline （PGI2） and thromboxane A2 （TXA2） levels in plasma were determined. At the same time, the activity of platelet aggregation was also determined. Results In rats treated with G-Rb （with dose of 50 and 100 mg · kg^-1 i. g for 7 d）, the MIS was significantly reduced （P〈0.05 or P〈0.01）, the AST, LDH and CK-MB activities in serum and the TXA2 level in plasma were declined （P〈0.05 or P〈0.01）, while PGI2 level in plasma and PGI2/TXA2 ratio were increased signficatly （P〈0.05 or P〈0.01）. In addition, the LPO content in serum was declined, SOD and GSH-Px activities in serum were increased markedly （P〈0.05 or P〈0.01）. The platelet aggregation rate （PAR） at 1, 3 and 5 rain and maximum platelet aggregation rate （MOAR） were decreased markedly （P〈0.05 or P〈0.01）. Conclusion G-Rb has protective effects on myocardial ischemia-reperfusion injury through improving free radicals metabolism, decreasing TXA2 level in plasma, increasing PGI2 level in plasma and PGI2/TXA2 ratio, and inhibitting the activity of platelet aggregation etc.

关 键 词：人参RB组皂苷 心肌缺血再灌注损伤 心肌酶 自由基 血管活性物质 血小板聚集功能 

分 类 号：R285.5[医药卫生—中药学] R542.2[医药卫生—中医学]