萝卜肉质根膨大过程中糖含量及蔗糖代谢相关酶活性分析  

Changes of Sugar Content and Sucrose Metabolizing Enzyme Activities During Fleshy Taproot Development in Radish(Raphanus sativus L.)

作　　者：王玮[1] 龚义勤[1] 柳李旺[1] 王燕[1] 荆赞革[1] 黄丹琼[1] 汪隆植[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室园艺学院,南京210095

出　　处：《园艺学报》2007年第5期1313-1316,共4页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30571193);江苏省自然科学基金项目(创新人才BK2004418);江苏省高技术研究计划项目(BG2005314)

摘　　要：用根冠比差异显著的萝卜品系为材料,研究了肉质根膨大过程中的若干生理生化变化。结果表明:4个品系肉质根的库活性变化情况基本一致,"破肚"后一周内达到最大;可溶性总糖、果糖和蔗糖含量初期下降,"破肚"期降至最低值,其中低根冠比品系"NAU-LR-04"蔗糖含量一直高于其它高根冠比品系,而其果糖含量则相对较低。肉质根中蔗糖合酶的活性变化与肉质根库活性的变化趋势基本一致,且低根冠比品系蔗糖合酶活性相对高于高根冠比品系,推测蔗糖合酶可能是调节肉质根蔗糖水平、控制肉质根库活性的关键酶。　Several physiological changes during the development of radish fleshy taproot were studied using the advanced inbred lines of different ratio of root to shoot（R/S）.The results showed that sink activity patterns of taproot were much similar among the four lines. The contents of carbohydrates including soluble total sugar, sucrose and fructose decreased at the beginning of taproot enlarging, and to the minimum at the stage of the primary cortex split. The sucrose content of the low R/S line NAU-LR-04 was higher than those of three high R/S lines at all stages, while the fructose content was lower in NAU-LR-04. Changes of activities of sucrose synthase（SuSy）were similar to sink activities in all lines, and the activity of SuSy in low R/S line was higher than those of high R/S lines. It can be inferred that the SuSy may play an important role in the sucrose level regulation and sink activity control during radish fleshy taproot thickening.

关 键 词：萝卜 肉质根 糖含量 蔗糖合酶 

分 类 号：S631.1[农业科学—蔬菜学]