维持性血液透析患者外周血调节性T细胞的表达  

Regulatory T cells in peripheral blood of maintenance hemodialysis patients

作　　者：眭维国[1] 孙燕燕[1] 黄河[1] 陈洁晶[1] 蓝慧娟[1] 戴勇[1] 

机构地区：[1]解放军第181医院全军肾移植与血液净化中心,桂林541002

出　　处：《中国血液净化》2007年第9期484-486,共3页Chinese Journal of Blood Purification

摘　　要：目的探讨维持性血液透析患者体内调节性T细胞表达水平及意义。方法采用流式细胞术测定解放军第181医院全军肾移植与血液净化中心13例维持性血液透析患者血液透析前、血液透析后外周血CD4+T细胞占淋巴细胞的比例及CD4+CD-127Treg、CD4+CD25+CD127-Treg占CD4+T细胞的比例。结果维持性血液透析患者血液透析前CD4+T细胞占淋巴细胞的比例高于健康对照组,CD4+CD127-Treg、CD4+CD25+CD127-Treg占CD4+T细胞的比例低于健康对照组,血液透析后CD4+T的比例下降,CD4+CD25+CD127-Treg的比例有所上升。结论尿毒症患者外周血CD4+T数量增高,CD4+CD127-Treg、CD4+CD25+CD127-Treg数量降低,免疫功能紊乱,而血液透析有利于改善机体的免疫功能。Objective To investigate the significance of CD4^＋CD127^-regulatory T cells in peripheral blood of maintenance hemodialysis patients. Methods The peripheral blood samples were collected from 13 maintenance hemodialysis patients, before trans-analysis and after. The CD4^＋CD127^-Treg and CD4^＋CD25^＋CD 127^-Treg ratios in CD4^＋T were detected by using flow cytometry. Results The CD4^＋T ratios in lymphocyte of maintenance hemodialysis patients were higher than healthy controls group before trans-analysis and the CD4^＋CD 127^-Treg and CD4^＋CD25^＋CD 127^-Treg ratios in CD4^＋T were lower, but CD4^＋T ratios decreased and CD4^＋CD25^＋CD 127^-Treg ratios increased after trans-analysis. Conclusion The number of CD4^＋T is increasing, CD4^＋CD 127^-Treg is decreasing in maintenance hemodialysis patients and their immune is disturbing, and hemodialysis can improve immune function of the patients.

关 键 词：尿毒症 血液透析 CD4^+CD127-调节性T细胞 流式细胞仪 

分 类 号：R318.16[医药卫生—生物医学工程]