水飞蓟素脂质体对BCG+LPS引起的小鼠免疫性肝损伤的保护作用  

The protective effect of silymarin liposomes against experimental liver injury in mice

作　　者：许红霞[1] 刘玲 王梅[1] 刘耕 高晓黎[1] 

机构地区：[1]新疆医科大学药学院 [2]新疆特丰药业股份有限公司,新疆乌鲁木齐830054

出　　处：《新疆医科大学学报》2007年第8期790-793,共4页Journal of Xinjiang Medical University

基　　金：自治区高技术研究发展计划资助项目(200311107)

摘　　要：目的：研究水飞蓟素脂质体（L-SIL）对卡介苗（BCG）＋脂多糖（LPS）引起的小鼠免疫性肝损伤的保护作用。方法：昆明种小鼠80只随机分为空白组、模型组及L-SIL低、中、高剂量组和益肝灵（T-SIL）低、中、高剂量组共8组,测量并比较各组小鼠血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）水平和肝组织匀浆中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）水平以及丙二醛（MDA）、一氧化氮（NO）含量,观察肝脏、脾脏系数的变化,并观察各组小鼠肝组织病理学改变。结果：与模型组相比,L-SIL各剂量组血清ALT、AST水平明显降低（P〈0.05-0.01）,肝组织匀浆GSH-PX、SOD水平明显升高（P〈0.05-0.01）,MDA和NO含量明显降低（P〈0.01）,肝脏、脾脏系数明显降低（P〈0.05-0.01）。L-SIL对ALT、AST、GSH-PX水平和NO含量的作用强于同剂量T-SIL组（P〈0.05-0.01）。组织学观察L-SIL低、中剂量组的肝损伤明显轻于模型组和相应剂量T-SIL组。结论：L-SIL对小鼠BCG＋LPS引起的免疫性肝损伤有明显的保护作用,且其保护作用明显优于T-SIL。Objective： To investigate the protective effect of silymarin liposomes （L-SIL） on the BCG＋LPS- induced liver injury in mice compared with silymarin tablets （T-SIL）. Methods： The models of hepatic damage were induced by BCG＋ LPS. The alanine aminotransferase （ALT）, aspartate aminotransferase （AST） activities in serum, malondialdehyde （MDA）, nitric oxide （NO） contents, superoxide dismutase （SOD）, glutathione peroxidase （GSH-PX） levels in liver homogenate, coefficient of liver were examined and dimensions of hepatic damage in the histopathology were observed. Results： L-SIL could inhibit the rising of serum ALT, AST activities caused by BCG＋LPS. Meanwhile, L-SIL also decreased MDA, NO contents, reduced the coefficient of liver and prevented the reduction of SOD, GSH-PX activities in the liver homogenate. The effects of L-SIL on the AI.T, AST activity in serum,GSH-PX activities and NO content in liver homogenate against experimental liver injury in mice were more powerful than that of T-SIL of the same dose. The liver pathological observation showed that the liver injuries of L-SIL （75,150 mg · kg^-1 · d^-1） were significantly less than that of the model group and T-SIL of the same dose. Conclusion： The protective effect of L-SIL on the BCG＋LPS-induced liver injury in mice was more potent than that of the same dose of T SII.

关 键 词：水飞蓟素脂质体 益肝灵片 肝损伤 

分 类 号：R975[医药卫生—药品] R965.1[医药卫生—药学]