膀胱梗阻大鼠逼尿肌不稳定与三磷酸肌醇关系的实验研究  

Experimental study of relationship between detrusor instability secondary to bladder outlet obstruction and IP3 in rats

作　　者：王军[1] 梁颖[2] 庄仕华[3] 李彤[1] 宋波[4] 金锡御[4] 叶章群[5] 

机构地区：[1]武警新疆总队医院泌尿外科,新疆乌鲁木齐830000 [2]新疆医科大学公共卫生学院营养与食品卫生教研室,新疆乌鲁木齐830054 [3]武警新疆总队医院肝胆外科,新疆乌鲁木齐830000 [4]第三军医大学西南医院全军泌尿外科研究所,重庆400038 [5]华中科技大学同济医学院附属同济医院泌尿外科,湖北武汉430030

出　　处：《新疆医科大学学报》2007年第8期803-806,共4页Journal of Xinjiang Medical University

摘　　要：目的:探讨膀胱梗阻大鼠逼尿肌不稳定(detrusor instability,DI)与三磷酸肌醇[inositol(1,4,5)-trisphosphat,IP3]含量变化的关系。方法:建立DI大鼠模型,分别检测DI大鼠、逼尿肌稳定(detrusor stability,DS)大鼠及经卡巴可(Carbachol)处理的DS大鼠逼尿肌原代培养细胞IP3含量。结果:DI大鼠逼尿肌原代培养细胞IP3含量显著高于DS大鼠(P<0.01),经10-5mmol/L卡巴可刺激后,DS大鼠逼尿肌原代培养细胞IP3含量较刺激前显著升高(P<0.01)。结论:肌醇脂质信号传导通路可能参与了逼尿肌不稳定的形成,IP3含量的升高可能在其中起着重要作用。Objective： To investigate the relationship between detrusor instability secondary to bladder outlet obstruction and the content change of inositol （1,4,5）-trisphosphate （IP3） in rats. Methods： To set up the model of detrusor instability （DI） of rats, IP3 contents of detrusor cultured cell in DI rats, detrusor stability （DS） rats which stimulated by 10^-5M carbachol in DS rats were detected. Results： IPa contents of detrusor cultured cell of DI rats were significantly higher than those of DS rats （P 〈0.01）, IP3 contents of DS rats detrusor cultured cell stimulated by 10-SM carbachol were also obviously higher than those of DS rats no stimulated （P d0.01）. Conclusions： Inositol-lipid signal transduction pathway might participate the pathogenesis of DI and the increasing of IP3 contents might play a important role .

关 键 词：三磷酸肌醇 逼尿肌不稳定 大鼠 

分 类 号：R691.2[医药卫生—泌尿科学] R694.2[医药卫生—外科学]