邻苯二甲酸二乙基己酯对小鼠不同脏器DNA的损伤作用  

Damage effects of Di-(2-ethylhexyl)phthalate(DEHP) on DNA in cells from various organs of mice

作　　者：王黎明[1] 丁书茂[1] 吴扬[1] 吴凯[1] 杨光涛[1] 娄小华[1] 李艳[1] 袁均林[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2007年第3期440-443,共4页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金资助项目(30570799).

摘　　要：应用单细胞凝胶电泳技术(SCGE)研究了邻苯二甲酸二异辛酯[Di-(2-ethylhexyl)phthalate,DEHP]对小鼠不同脏器(睾丸、胃、脑和肝)DNA的损伤作用.结果表明,DEHP可引起小鼠DNA的损伤,并且随着DEHP浓度的逐渐增高,DNA的损伤也随之加重,它们之间具有明确的剂量效应关系.在睾丸的细胞,胃内膜的细胞,脑的细胞中,当DEHP的浓度达到375 mg/kg时,则可造成DNA的极显著断裂(P<0.01).而在肝的细胞中,当DEHP的浓度达到125 mg/kg时,就可造成DNA的极显著断裂(P<0.01).当DEHP的浓度达到500 mg/kg时,除了脑的细胞外,DNA的断裂均不显著,可能已形成交联.Single cell gel electrophoresis （SCGE） was used in the present study to detect the DNA damage induced by Di-（2-ethylhexyl） phthalate （DEHP） in various organs （testis, stomach, brain and liver） of mice. The results showed that DEHP could induce DNA damage in mice, and the damage effect was increased with the concentrations of DEHP, indicating the dose-dependent manner of DEHP toxicity. A significant DNA breakage was observed in the cells of testis, stomach and brain was the concentration of DEHP was 375 mg/kg （p〈0.01）. Meanwhile, DEHP at concentration of 125 mg/kg could induce significant DNA breakage in the cells of liver （p〈0.01）. When the concentration of DEHP was 500 mg/kg, DNA breakage was not significant except in brain cells, which may be probably resulted from the formation of crosslinks.

关 键 词：邻苯二甲酸二异辛酯 DNA 器官 小鼠 彗星实验 

分 类 号：Q523[生物学—生物化学]