抗心律失常肽对乳鼠心肌细胞急性缺氧时细胞间传导的影响  

Effects of Antiarrhythmic Peptide on Intercelluar Communication in Cultured Neonatal Rat Myocytes during Acute Hypoxia

作　　者：王荣[1] 张存泰[1] 刘念[1] 阮燕菲[1] 王琳[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院心内科,武汉430030

出　　处：《华中科技大学学报（医学版）》2007年第5期574-577,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No30370573)

摘　　要：目的研究抗心律失常肽(AAP10)对乳鼠心肌细胞急性缺氧时细胞间传导的影响。方法原代培养的乳鼠心肌细胞,随机分为正常组、缺氧2h组和100nmol/LAAP10干预组,每组n=9。采用划痕标记染料示踪技术测定细胞间通讯,Western blot技术检测缝隙连接蛋白43(Connexin43,Cx43)磷酸化水平的改变。结果划痕标记染料示踪技术结果显示:缺氧组细胞间传导下降,而AAP10干预后能促进荧光黄的扩散。Western blot结果显示:缺氧组总Cx43蛋白表达下降,与正常组比较P<0.01,而去磷酸化的Cx43(NP-Cx43)蛋白表达不变;AAP10干预组能提高总Cx43蛋白表达,与缺氧组比较P<0.01,但对NP-Cx43蛋白表达无影响。结论急性缺氧时磷酸化的Cx43(p-Cx43)蛋白表达下降,而AAP10改善传导主要通过促进Cx43磷酸化。Objective To investigate the effects of the antiarrhythmic peptide AAP10 on the conduction of cultured neonatal rat ventricular myocytes（NRVMs） during acute hypoxia.Methods NRVMs were randomly divided into 3 groups：control group,hypoxia group and AAP10 group（n=9 each）.The intercellular communication was evaluated by the scrape loading assay.The state of Cx43 phosphorylation was examined by Western blot.Results The results of scrape loading indicated that the conduction of gap junction was decreased during acute hypoxia,while AAP10 could promote the diffusion of Lucifer Yellow.Western blot analysis showed that total Cx43 was greatly decreased during hypoxia（P〈0.01）,while dephosphorylated Cx43（NP-Cx43） was unchanged.AAP10 inhibited the decrease of total Cx43（P〈0.01）,but had no effect on the NP-Cx43.Conclusion During acute hypoxia,phosphorylated Cx43（p-Cx43） was reduced.AAP10 could improve intercelluar communication by enhancing phosphorylation of Cx43.

关 键 词：缺氧 缝隙连接蛋白43 磷酸化 抗心律失常肽 

分 类 号：R541.7[医药卫生—心血管疾病]