改进荧光光谱法研究药物与血清白蛋白的相互作用  

Studies on Interaction between Drugs with Serum Albumin by Modified Fluorescence Spectroscopy

作　　者：谭韬[1] 黄锐[1] 夏之宁[1] 

机构地区：[1]重庆大学化学化工学院,重庆400030

出　　处：《分析化学》2007年第10期1415-1420,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20375051);教育部跨世纪人才培养计划(No.教技函[2001]3号)资助项目

摘　　要：为了解决蛋白与药物分子的荧光光谱相互作用分析存在的缺点,采用小分子为荧光检测对象,以血清白蛋白(SA)为猝灭剂,研究了模拟生理条件(pH=7.4)下伯氨喹、十二烷基苯磺酸钠和西酞普兰与SA的相互作用。测得伯氨喹、十二烷基苯磺酸钠和西酞普兰与SA的结合常数Kb分别为8.16×104L/mol、4.14×106L/mol和6.08×107L/mol。这种改进荧光光谱法能更准确和全面地表达出SA与药物的相互作用信息。通过紫外吸收光谱相互作用分析法对改进方法进行了验证。并推测出药物与蛋白之间存在一种"点对面"的作用方式。To resolve the deficiencies in the interaction analysis by classical fluorescence spectroscopy between serum albumin （SA） and drug, a modified fluorescence spectroscopy has been applied to study the binding constant （Kh） of the interaction between SA and primaquine, sodium dodecyl benzene sulfonate and citalopram under simulated physiological conditions （pH = 7.4） , in which the fluorescence detective object is small molecule drugs and the quencher is SA. The values of Kb obtained were 8.16 × 10^4 L/mol, 4.14 × 10^6 L/mol and 6.08 × 10^7 L/mol, respectively. The rationality and correctness in the modified method has been proved based on Ultra-Violet absorption spectroscopy. The results show that this modified method can reveal the more and true information of some interactions. Thus the interaction mode between drugs and SA included point-to-area has been drawn.

关 键 词：荧光光谱法 牛血清白蛋白 相互作用 伯氨喹 西酞普兰 十二烷基苯磺酸钠 

分 类 号：R96[医药卫生—药理学]