液相色谱和质谱联用技术结合化学计量学应用于代谢组学的研究进展  

Advances of Liquid Chromatography/Mass Spectrometry Combined with Chemometric Approaches Applied to Metabonomics

作　　者：林艳萍[1] 司端运[2] 刘昌孝[2] 

机构地区：[1]天津大学化工学院制药工程系,天津300072 [2]天津药物研究院天津药代动力学与药效动力学省部共建国家重点实验室,天津300193

出　　处：《分析化学》2007年第10期1535-1540,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20675056;30630075)资助项目

摘　　要：代谢组学作为系统生物学的重要组成部分,已经成为继基因组学、转录组学、蛋白质组学之后兴起的一个新的组学研究热点。准确全面的检测生物体液中浓度较低的代谢物变化是进行代谢组学研究的基础。液相色谱和质谱联用(LC/MS)技术结合化学计量学很好地实现了对大量样品和微量代谢物的快速定性、定量分析,极大地推动了代谢组学的相关研究。本文综述了LC/MS与化学计量学相结合用于代谢组学研究的现状,并对后续的研究进行了展望。Within the past few years, metabonomics, as a newpart of system biology, has gained great prominence besides genomics, transcriptomics and proteomics. Thus, it is necessary to detect in vivo metabolites thoroughly and exactly, which is traditionally considered as small endogenous compounds at trace levels, from complex biomatrice as the basic methodological considerations. Liquid chromatography coupled to mass spectrometry （LC/MS） combined with chemometric methods acted as a prominent process to accelerate the development of metabonomics, because of their promising ability in qualitative and quantitative analysis of traue amount of metabolites in complicated samples . The strategies of LC/MS combining chemometric methods and the actualities of metabonomic studies, with the prospective development of this research subject, were discussed in this article.

关 键 词：液相色谱和质谱联用技术 化学计量学 代谢组学 评述 

分 类 号：O657.63[理学—分析化学]