异氟醚对大鼠海马脑片突触长时程增强的影响  

Effect of isoflurane on synaptic long-term potentiation in rat hippocampal slices

作　　者：冯春生[1] 徐海洋[1] 麻海春[1] 张永谦[2] 岳云[2] 

机构地区：[1]吉林大学白求恩医学部附属第一医院麻醉科,长春市130021 [2]首都医科大学附属北京朝阳医院麻醉科

出　　处：《中华麻醉学杂志》2007年第10期896-900,共5页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金资助项目（30471665）;中国博士后科学基金资助项目（20040350370）

摘　　要：目的评价异氟醚对大鼠海马脑片突触长时程增强（LTP）的影响。方法雄性SD大鼠,断头后取出海马组织,制备厚400μm的海马脑片。采用细胞外微电极记录技术,记录海马脑片CA1区细胞外群体峰电位（PS）。取28张脑片,随机分为4组（n=7）：用正常的人工脑脊液（ACSF）灌流海马脑片,记录正常的PS,待其稳定后,对照组继续灌流ACSF,不同浓度异氟醚组分别用含异氟醚0.125 mmol/L（异氟醚0.125组）、0.25 mmol/L（异氟醚0.25组）、0.5 mmol/L（异氟醚0.5组）的ACSF灌流,记录PS幅值。另取70张脑片,随机分为10组（n=7）：用正常ACSF灌流海马脑片,记录稳定正常的PS 30 min,LTP组继续灌流ACSF,其余各组分别用含异氟醚0.125 mmol/L（异氟醚LTP 0.125组）、0.25 mmol/L（异氟醚LTP 0.25组）、0.5 mmol/L（异氟醚LTP 0.5组）、印防己毒素0μmol/L（印防己毒素组）、荷包牡丹碱10μmol/L（荷包牡丹碱组）、CGP353485μmol/L（CGP35348组）、印防己毒素50μmol/L＋异氟醚0.25 mmol/L（印防己毒素＋异氟醚组）、荷包牡丹碱10/μmol/L＋异氟醚0.25 mmol/L（荷包牡丹碱＋异氟醚组）、CGP353485μmol/L＋异氟醚0.25 mmol/L（CGP353485＋异氟醚组）的ACSF灌流,记录PS 30 min后,施以100 Hz的高频强直刺激（HFS）,记录PS幅值。结果与对照组比较,异氟醚0.125组给药后10～45 min PS幅值降低,异氟醚0.25组和异氟醚0.5组给药后5～45 min PS幅值降低（P〈0.05或0.01）。LTP组HFS后5～60 min PS幅值增高,较刺激前增加了（52±12）%（P〈0.01）。与HFS前比较,异氟醚LTP 0.125组、异氟醚LTP 0.25组和异氟醚LTP 0.5组给予HFS后PS幅值差异无统计学意义（P〉0.05）;与LTP组比较,3组PS幅值降低（P〈0.01）。与HFS前比较,印防己毒素组、荷包牡丹碱组和CGP 35348组HFS后PS幅值增加（P〈0.01）;与LTP组比较,3组PS幅值差异无统计学意义（P〉0.05）。与HFS前比较,印防己毒素＋异氟醚组和荷包牡丹�　Objective To investigate the effect of isoflurane on the synaptic long-term potentiation（LTP） in the CA1 area of rat hippocarnpal slices and the underlying mechanism.Methods Hippocampal slices（400μm thick）were prepared from the brains of male 2-month old SD rats weighing 200-250 g anesthetized with ether and decapitated.The slices were incubated in artificial cerebrospinal fluid（ACSF）at room temperature for at least 120 min before use.Twenty-eight slices were randomly divided into 4 groups（n=7 each）：in groupⅠthe slices were perfused with ACSF（control）and in groupⅡ,ⅢandⅣthe slices were perfused with ACSF containing isoflurane 0.125,0.25 and 0.5 mmol/L respectively to determine the effect of different concentrations of isoflurane on the baseline amplitude of evoked population spikes（PS）.Another 70 slices were randomly divided into 10 equal groups（n=7 each）：ⅠLTP group in which the slices were perfused with ACSF;in groupⅡ,Ⅲ,Ⅳthe slices were perfused with ACSF containing isoflurane 0.125,0.25 and 0.5 mmol/L respectively;group V picrotoxin 50μmol/L;groupⅥbicuculline 10μmol/L;groupⅦCGP35348 5μmol/L;groupⅧpicrotoxin 50μmol/L＋ isoflurane 0.25 mmol/L ;groupⅨbicuculline 10μmol/L＋isoflurane 0.25 mmol/L and groupⅩCGP35248 5μmol/L＋isoflurane 0.25 mmol/L.PSs were recorded for at least 30 min before LTP in each group.For LTP induction,high-frequency stimulation（HFS）（100 Hz/S）was applied to the Schaffer collateral-commissural pathway of hypocampus using a stimulating electrode.The changes in PS amplitude after HFS were analyzed in each group.Results（1）The PS amplitude in the 3 isoflurane groups was significantly decreased dose- dependently compared with the control.（2）The PS amplitude after HFS increased by（52±12）% as compared with that before HFS indicating successful LTP induction in LTP group（groupⅠ）.（3）The PS amplitude after HFS did not change significantly in the 3 isoflurane groups（groupⅡ,Ⅲ,Ⅳ）and was significan

关 键 词：异氟醚 海马 突触 长时程增强 

分 类 号：R686[医药卫生—骨科学]