雅培CD3700测定白细胞线性范围的验证与ISO15189应用  

Certification of linear range of WBC measured by CD3700 and application in ISO15189

作　　者：杨有业[1] 杨志钊[1] 缪丽韶[1] 黄福达[1] 梁锦胜[1] 

机构地区：[1]中山市人民医院,广东中山528403

出　　处：《基础医学与临床》2007年第10期1165-1167,共3页Basic and Clinical Medicine

摘　　要：目的验证雅培CD3700血细胞计数仪测定血液白细胞的线性范围。方法用浓缩白膜层制备高值白细胞标本,以生理盐水稀释成系列浓度标本,每个浓度测定2次,各浓度的测定顺序随机,对数据进行离群值检验、回归分析和随机误差分析。结果测定结果无离群值,经回归检验在(0～219)×109L-1范围内为一条通过0点的直线,直线的随机误差小于1/4CLIA’88允许误差范围(3.75%)。结论CD3700测定血液白细胞的线性范围与该型号仪器出厂标准一致。Objective To certify the linear range of WBC measured by CD3700. Methods Use the white stratum of blood after centrifugation. Then, the high value specimens were diluted with saline to get a series of samples with different concentrations. Every specimen was measured twice in order to keep the sequence of specimens randomly. The perform outlier test, polynomial regression analysis, and the random error estimation were carried out. Results There was no outher in the results. It was demonstrated by polynomial regression to be linear from （0 - 219） × 10^9 L^-1 The random error was within the allowable limit of 1/4CLIA＇88 （3.75%）. Conclusion The linear range of WBC measured by CD3700 is just like that of the manufacturer claims.

关 键 词：白细胞 线性范围 验证 

分 类 号：R392[医药卫生—免疫学]