硬膜外腔植入髓核对大鼠脊髓背根神经节TNF-α和IL-1表达的影响  

Effect of nucleus pulposus allografted into epidural space on the expressions of TNF-α and IL-1 in rat′s dorsal root ganglion neurons

作　　者：张挺杰[1] 倪家骧[1] 张春雷[1] 李娟红[1] 于翠萍[1] 

机构地区：[1]首都医科大学宣武医院疼痛诊疗科,100053

出　　处：《中国康复医学杂志》2007年第10期897-898,903,共3页Chinese Journal of Rehabilitation Medicine

基　　金：北京市科技计划项目(Y0204003040631)

摘　　要：目的:观察硬膜外腔植入异体髓核对大鼠L6-S1脊髓背根神经节细胞TNF-α和IL-1表达的影响,以期为椎间盘源性疼痛发病机制提供细胞生物学基础。方法:雄性SD大鼠(体重260—280g)随机分为4组:脂肪+生理盐水组(FS组)、脂肪+胶原酶组(FE)、髓核+生理盐水组(NS)、髓核+胶原酶组(NE组),每组6只。术后第15天取L6-S1脊髓背根神经节,采用免疫组织化学染色方法观察髓核对背根神经节细胞炎性介质TNF-α和IL-1表达的影响。结果:术后15天FE组大鼠与FS组相比,背根神经节细胞TNF-α表达差异没有显著性意义(P>0.05);NS组与FE组相比背根神经节细胞TNF-α表达显著增加(P<0.05);NE组与NS组相比背根神经节细胞TNF-α表达显著降低(P<0.05),接近于FS组水平(P>0.05)。IL-1在各组大鼠背根神经节中未染色。结论:硬膜外腔植入异体髓核可引起背根神经节细胞TNF-α表达增加。Objective： To observe the effect of nucleus pulposus allografted into the epidural space on the expressions of TNF-αand IL-1 in rat＇s DRG neurons, so as to provide theoretical evidence for discogenic pain. Method： Male Sprague-Dawley rats were randomly divided into four groups with 6 rats in each： fat ＋ nature saline group（FS group）, fat＋collagenase group （FE group）, nucleus pulposus ＋ nature saline group（NS group）, and nucleus pulposus＋collagenase group （NE group）. The allografted fat and nucleus pulposus were provided by other 8 male SD rats. 15 days after surgery, the L6-S1 DRGs were seperated, and the expressions of TNF-α and IL-1 in DRGs were detected by using immunohistochemistry method. Result： 15 days after surgery, the expressions of TNF-α in DRGs in NS group was stronger than FE group （P〈0.05）; but the expressions of TNF-α in NE goup was less than NS without significant difference compared with FS group （P〉0.05）. Conclusion： Nucleus pulposus allografted into the epidural space can increase the TNF-α expressions in rat＇s DRG.

关 键 词：髓核 背根神经节 肿瘤坏死因子-α 白细胞介素-1 

分 类 号：R681.53[医药卫生—骨科学]