增殖抑制基因在原发性高血压患者血管平滑肌细胞增殖中的作用  

Hyperplasia suppressor gene expression in vascular smooth muscle cells derived from normotentive and hypertensive patients underwent bypass surgery

作　　者：刘亚萍[1] 温绍君[1] 刘雅[1] 赵莉敏[1] 郭艳红[2] 陈新军[1] 王佐广[1] 刘洁琳[1] 文杰[1] 王世奇[1] 汤健[2] 

机构地区：[1]首都医科大学附属北京安贞医院 [2]北京大学医学部

出　　处：《中华心血管病杂志》2007年第10期914-918,共5页Chinese Journal of Cardiology

基　　金：北京市自然科学基金(7073089)

摘　　要：目的探讨增殖抑制基因(HSG)在原发性高血压患者血管平滑肌细胞增殖中的作用。方法选取2006年4月至9月在北京安贞医院行冠状动脉旁路移植术的患者54例,分为高血压冠状动脉旁路移植组(n=28)和正常血压冠状动脉旁路移植组(n=26)。术前留取两组患者静脉血,测定血液生化指标,用放射免疫测定法测定内皮素(ET)和肾素-血管紧张素-醛固酮系统等血管活性多肽;用 Trizol 一步法提取白细胞、组织和培养血管平滑肌细胞(VSMC)的总 RNA,行半定量 RT-PCR 扩增反应,观察两组患者 HSG 的表达情况。结果两组指标的血压和体质指数(BMI)的差异具有统计学意义(P<0.01)。BMI 与血糖、BMI 与收缩压、BMI 与舒张压、血糖与甘油三酯、收缩压与舒张压之间存在着明显的正相关(P<0.05)。五种血管活性物质均存在高血压搭桥组患者高于正常血压搭桥组患者的趋势,差异没有统计学意义。白细胞、血管组织和 VSMC 中 HSG 的表达在高血压搭桥组较正常血压搭桥组低(P<0.05)。相关性分析表明:血管 HSG 表达与 BMI、收缩压、舒张压之间存在着明显的负相关(P<0.05)。结论在高血压冠心病患者和正常血压冠心病患者之间,无论在白细胞、VSMC 还是在血管组织,都存在 HSG 表达差别,高血压组的 HSG 表达水平均低于正常血压组。在血管组织中,HSG 的表达与 BMI、收缩压和舒张压呈负相关。说明 HSG 是一种新型的抑制血管平滑肌细胞增殖的基因,其在血管平滑肌中的表达与血压呈负相关。Objective To investigate the novel hyperplasia suppressor gene （HSG） expression in vascular smooth muscle cells derived from normotensive and hypertensive patients underwent bypass surgery. Methods Coronary heart disease patients underwent coronary artery bypass graft （CABG） operation in BEIJING ANZHEN hospital from 4-9, 2006 were enrolled in this study and divided into hypertensive group （n = 28） and normotensive group （n = 26）. The preoperative venous blood samples were taken for serum biochemical and vasoactive peptides measurements. Total RNA was extracted from WBC, explanted-vessels and cultured VSMCs using TRIZOL and HSG expression was determined by Semi- Quantitative RT-PCR. Results Body mass index （BMI） was significantly higher in hypertensive group compared to normotensive group （P 〈 0. 01 ） while other biochemic parameters and vasoactive peptides were similar between the groups. BMI and GLU, BMI and SBP, BMI and DBP, GLU and TG, SBP and DBP were positively correlated （all P 〈 0. 05）. HSG expression in WBC, VSMCs and vessel tissue were significantly lower in hypertensive group than those in normotensive group （ all P 〈 0. 05）. HSG expression in tissue was negatively correlated to BMI, SBP and DBP （all P 〈 0. 05 ）. Conclusions Reduced HSG expression and the negative correlation on vascular tissue HSG expression to BMI, SBP and DBP suggested a possible inhibitory role of HSG on VSMC proliferation and blood pressure.

关 键 词：高血压 基因 肌 平滑 血管 

分 类 号：R544.1[医药卫生—心血管疾病]