磷脂酰肌醇3激酶通路在胰岛素调节子宫内膜癌细胞增殖凋亡中的作用  

Role of phosphatidylinositol 3-kinase signal pathway in insulin-induced regulation of proliferation and apoptosis in endometrial cancer cells

作　　者：华绍芳[1] 薛凤霞[1] 赵敬[1] 

机构地区：[1]天津医科大学总医院妇产科

出　　处：《中国实用妇科与产科杂志》2007年第11期843-845,共3页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：国家自然科学基金(30471810)

摘　　要：目的探讨磷脂酰肌醇3激酶(PI3K)信号通路在胰岛素调节子宫内膜癌细胞生长中的作用。方法2006年5月至2006年10月于中国医学科学院血液病学研究所临床药物室完成试验,即将子宫内膜癌Ishika-wa3-H-12细胞进行无血清饥饿培养,分成不同处理组,即空白对照组、胰岛素刺激组(Ins组)、PI3K抑制剂——LY294002预处理后再以胰岛素刺激组(LY+Ins组)。以四甲基偶氮唑蓝(MTT)试验和流式细胞仪观察细胞增殖和凋亡情况。不同处理组分别培养24h、48h和72h,观察各组570nm波长吸光度(OD570nm)。结果Ins组细胞OD570nm值高于空白对照组(P<0.01);LY+Ins组细胞OD570nm低于Ins组(P<0.01),LY294002拮抗了胰岛素对内膜癌细胞的增殖促进作用。流式细胞仪检测凋亡发现:Ins组细胞凋亡率低于空白对照组[(2.02±0.39)%对(14.46±2.70)%,P<0.01];LY+Ins组细胞凋亡率高于Ins组[(18.73±2.11)%对(2.02±0.39)%,P<0.01]。结论PI3K信号通路在胰岛素引起的促内膜癌细胞增殖、抑制凋亡中起重要作用,胰岛素对子宫内膜癌细胞增殖、凋亡的调节作用是PI3K依赖性的。Objective To explore the role of phosphatidylinositol 3-kinase （PI3K） signal pathway in insulin-induced proliferation promotion and apoptosis inhibition in endometrial cancer cells. Methods Endometfial cancer cell line Ish-ikawa3-H-12 was starve-cultured in serum-free medium, The cells were divided into different groups ： control group, the group stimulated by insulin alone（ Ins）, the group preincubated with PI3K inhibitor LY294002 prior to insulin treatment （ LY ＋ Ins）. We observed the proliferation and apoptosis of endometrial cancer cells by MTr assay and flow cytometric analysis. Results The absorbance at 570nm（OD570nm） in different groups at 24h, 48h and 72h after treatment was measured. Mter,insulin treatment, the OD570nm in these three time points were higher than the controls （ all P 〈 0. 01 ）. However, OD570nm in LY ＋ Ins group was lower than the group treated with insulin alone （P 〈 0. 01 ）. The apoptotic rate of Ins group was lower than the control[ （ 2.02 ± 0. 39 ） % vs （ 14. 46 ±2. 70） %, P 〈 0. 01 ]. When co-treated with LY294002 and insulin, the apoptotic rate was higher than the group treated with insulin alone. Conclusion Proliferation promotion and apoptosis inhibition induced by insulin in endometrial cancer cells are mediated through PI3K signal pathway.

关 键 词：子宫内膜癌 胰岛素 磷脂酰肌醇3激酶 增殖 凋亡 

分 类 号：R71[医药卫生—妇产科学]