高效液相色谱法测定生长抑素含量及有关物质  

RP-HPLC Determination of Simvastatin and Its Related Substance Lovastatin

作　　者：尹海滨[1] 郑虎[1] 

机构地区：[1]四川大学华西药学院,成都610041

出　　处：《中国现代应用药学》2007年第5期388-390,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立高效液相色谱测定生长抑素含量的方法。方法采用Ominispher C18柱（250 mm×4.6 mm,5μm）,流动相A：0.1%TFA水溶液,流动相B：0.1%TFA乙腈溶液,采用梯度洗脱,检测波长：215 nm,流速1.0 mL.min-1。结果方法的线性范围为40-140μg.mL-1。r=0.999 9,平均回收率100.3%,最低检测限为1 ng。结论采用HPLC梯度洗脱法测定生长抑素的含量,分离度好,方法简便,适用于生长抑素的含量测定。OBJECTIVE To establish a HPLC method for determinating somatostatin content. METHODS The HPLC column was Ominispher C18 （250 mm × 4.6 mm,5 μm. varian corporation, USA）. The mobile phase A：0.1% TFA in water; the mobile phase B ：0.1% TFA in acetonitrile. The gradient elution method was used that mobile phase B was increase from 0% to 35% in twenty minutes. The flow rate was 1.0 mL · min^-1 and the detection wavelength was 215 nm. RESULTS There was a good liner relationship with the range of 40 - 140 μg · mL^-1（ r =0. 999 9）. The average recovery was 100.3% and the detect limit was 1 ng. CONCLUSION The method to determinate the content of somatostatin by HPLC was simple and reliable.

关 键 词：生长抑素 高效液相色谱法 梯度洗脱 含量测定 

分 类 号：R917.101[医药卫生—药物分析学] R977.1[医药卫生—药学]