高效液相色谱法测定寿星补汁中二苯乙烯苷的含量  

Determination of 2,3,5,4′-tetrahydroxy-tilbene-2-O-β-D-glucoside in Shouxing Buzhi by HPLC

作　　者：石钺[1] 郑顺亮[1] 曹阳[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094

出　　处：《中国现代应用药学》2007年第5期405-406,共2页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立寿星补汁中二苯乙烯苷的含量测定方法。方法采用HPLC,色谱柱为Kromasil C18（4.6 mm×250 mm,5μm）;流动相为乙腈-水（25∶75）;检测波长320 nm。结果二苯乙烯苷在0.01282-0.2564μg范围内与峰面积呈良好的线性关系,回归方程为Y=3.5303×106X-2424.9,r=0.999 9;平均回收率为99.44%,RSD为0.72%。结论本法测定寿星补汁中二苯乙烯苷的含量简便、准确、快捷,可用于寿星补汁的质量控制。　OBJECTIVE To establish a HPLC method for the determination of 2,3,5,4′-tetrahydroxy-tilbene-2-O-β-D-glucoside in Shouxing Buzhi.METHODS 2,3,5,4′-tetrahydroxy-tilbene-2-O-β-D-glucoside was separated on kromasil C18（4.6 mm×250 mm,5 μm） column and detected at 320 nm,with acetonitrile-water（25∶75） as a mobile phase. RESULTS There was good linearity from 0.01282 to 0.2564 μg（Y=3.5303×10^6X-2425.9,r=0.999 9） in separation of 2,3,5,4′-tetrahydroxy-tilbene-2-O-β-D-glucoside.The average recovery was 99.44% with RSD of 0.72%.CONCLUSION This method is simple,accurate and rapid.This study provides the reference to establish quality standard for Shouxing Buzhi.

关 键 词：高效液相色谱法 寿星补汁 2 3 5 4′-四羟基二苯乙烯-2-O--βD-葡萄糖苷 

分 类 号：R917.101[医药卫生—药物分析学] R289[医药卫生—药学]