高效液相色谱法测定急支糖浆中原儿茶酸的含量  

Determination of the Content of Somatostatin and Its Related Substances by HPLC

作　　者：王勤 张永玲 侯铁军 

机构地区：[1]甘肃省张掖市药品检验所,甘肃张掖734000 [2]甘肃省临夏州人民医院,甘肃临夏731100

出　　处：《中国现代应用药学》2007年第5期406-408,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立急支糖浆中原儿茶酸的含量测定方法。方法高效液相色谱法测定,Lichrosorb ODS C18（250 mm×4.6 mm,5μm）为色谱柱,以乙腈-水-冰醋酸（8∶100∶0.2）为流动相,检测波长为259 nm。结果原儿茶酸进样量在0.15-0.73μg范围内具有良好的线型关系,r=0.999 8,平均加样回收率为98.86%,RSD=0.70%（n=5）。结论所建立的定量方法准确、重复性好、专属性强,可作为控制和评价本品质量的方法。　OBJECTIVE To establish a HPLC-ELSD method for the determination of Astragaloside in Shenkangning Pian.METHODS The content of astragaloside Ⅳ was determined by HPLC-ELSD. RESULTS A good linearity was observed for astragaloside Ⅳ in the range of 1.036~7.77 μg.The average recovery of astragaloside Ⅳ was 98.6% with RSD=1.3%（n=11）.CONCLUSION HPLC-ELSD was simple and accurate with a good repeatability.It can be used for the quality control of Shenkangning Pian.

关 键 词：高效液相色谱法 急支糖浆 原儿茶酸 

分 类 号：R917.101[医药卫生—药物分析学] R283.6[医药卫生—药学]