Isolation and Characterization of a Phytotoxin from Xanthomonas campestris pv. retroflexus  

作　　者：李明智 徐凌 孙自伶 李永泉 

机构地区：[1]College of Life Science, Zhejiang University, Hangzhou 310058, China [2]Research Institute of Subtropical Forestry, China Academy of Forestry, Fuyang 311400, China

出　　处：《Chinese Journal of Chemical Engineering》2007年第5期639-642,共4页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Fotmdation of China （No.30370939）, Natural Science Foundation of Zhejiang Province （No.300054） and Science Research Plan of Zhejiang Province （No.2004C22005）.

摘　　要：A phytotoxin from Xanthomonas campestris pv. retroflexus was isolated using a chromatographer and HPLC, and the components were identified to be a mixture of minor molecular compounds including organic acids and cyclo-（proline-phenylalanine）. The greenhouse cultivation test was used to determine the influence of the isolated fractions on the growth of target weed redroot pigweed （Amaranthus retroflexus L）. The experimental results demonstrated that the cyclo-（Pro-Phe） had the weed inhibit activity obviously on dicotyledonous weed and the mixture with six organic acids showed stronger bioactivity. Further, greenhouse and field test were processed, and the test showed that the use of the toxin appeared to have the potential to be developed further as a bioherbicide system to control weedy grasses.从 Xanthomonas campestris pv 的 phytotoxin。反曲我们用 chromatographer 和 HPLC 被孤立，并且部件被识别是包括器官的酸和出租机动三轮车的次要的分子的混合物的混合物 --( 脯氨酸本氨基丙酸) 。温室耕作测试被用来在目标杂草红根黎的生长上决定孤立的部分的影响(Amaranthus 反曲我们 L ) 。试验性的结果证明出租机动三轮车 --(Pro-Phe ) 让杂草与六器官的酸在双子叶的杂草和混合上显然禁止活动显示出的更强壮的简历活动。进一步，温室和域测试被处理，并且测试证明毒素的使用看起来有潜力作为一个简历除草剂系统进一步被发展控制蔓延的草。

关 键 词：Xanthomonas campestris pv. retroflexus PHYTOTOXIN ISOLATION identification minor molecular compounds cyclo-（Pro-Phe） redroot pigweed BIOHERBICIDE 

分 类 号：Q949.3[生物学—植物学]