Integrative Extraction of Ergosterol, （1→3）-α-D-Glucan and Chitosan from Penicillium chrysogenum Mycelia  

作　　者：王天奇 李翰祥 王满意 谭天伟 

机构地区：[1]Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China

出　　处：《Chinese Journal of Chemical Engineering》2007年第5期725-729,共5页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China （No.20636010, No.50373003, No.20406002）, Beijing Natural Science Foundation （No.2071002）, and the Special Funds for Major State Basic Research Program of China （973 Program, No.2007CB714305）.

摘　　要：Abstract Ergosterol,（1→3）-α-D-glucan and chitosan are important biomaterials. In this research, a process has been developed to integratively extract ergosterol, （1→3）-α-D-glucan, and chitosan from Penicillium chrysongenum mycelium. First the mycelia are pretreated with 0.1mol·L^-1 of NaOH. After recovery by centrifugation the solid portion is made to undergo saponification and deacetylation reactaons by addition of 2mol·L^-1 NaOH and et anol.After reaction, extraction is carried out by addition of petroleum ether, which separates the reaction mixture into two phases. The upper layer of petroleum ether contains extracted ergosterol, and the .bottom layer of NaOH solution contains （1→3）-α-DEglucan; the chitosan is on the mycelia residuum. After isolation, the recovery yield of ergosterol is 0.52% of dry mycelium. That of （1→3）-α-D-glucan is about 8.2%; and chitosan is 5.7% with 86% deacetylation. The compositions have been characterized by 1R, HPLC analyses.Ergosterol,(1→3)-α-D-glucan and chitosan are important biomaterials.In this research,a process has been developed to integratively extract ergosterol,(1→3)-α-D-glucan,and chitosan from Penicillium chrysongenum mycelium.First,the mycelia are pretreated with 0.1mol·L-1 of NaOH.After recovery by centrifugation,the solid portion is made to undergo saponification and deacetylation reactions by addition of 2mol·L-1 NaOH and ethanol. After reaction,extraction is carried out by addition of petroleum ether,which separates the reaction mixture into two phases.The upper layer of petroleum ether contains extracted ergosterol,and the bottom layer of NaOH solu-tion contains(1→3)-α-D-glucan;the chitosan is on the mycelia residuum.After isolation,the recovery yield of er-gosterol is 0.52%of dry mycelium.That of(1→3)-α-D-glucan is about 8.2%;and chitosan is 5.7%with 86% deacetylation.The compositions have been characterized by IR,HPLC analyses.

关 键 词：ERGOSTEROL （1→3）-α-D-glucan CHITOSAN EXTRACTION Penicillium chrysogenum 

分 类 号：Q949.3[生物学—植物学]