通心络抑制兔血管外膜损伤致内膜动脉粥样硬化斑块及CD40表达  

Tongxinluo inhibits formation of vascular adventitia damage-induced atherosclerotic plaques and CD40 expression in rabbits

作　　者：刘永[1] 汤月霞[1] 梁春[1] 吴宗贵[1] 

机构地区：[1]第二军医大学附属长征医院心内科,上海200003

出　　处：《上海医学》2007年第10期734-736,F0004,共4页Shanghai Medical Journal

基　　金：国家重点基础研究发展计划(973计划)资助项目(2005CB523309)

摘　　要：目的观察通心络对兔血管外膜损伤致内膜动脉粥样硬化斑块及斑块CD40表达的影响。方法采用胶原酶消化法去除16只新西兰雄性大白兔左侧颈总动脉的外膜,术后予高脂饮食,并随机分为两组:通心络组以通心络(0.3 g·kg^(-1)·d^(-1))混悬液灌胃,对照组以等量蒸馏水灌胃。12周后取血及双侧颈总动脉标本,采用组织形态学方法检测动脉粥样斑块,免疫组织化学方法检测CD40表达。结果①两组实验前的胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)的差异均无统计学意义(P值均>0.05)。实验12周后,通心络组的TC、LDL-C均显著低于对照组(P值均<0.01),TG的差异无统计学意义(P>0.05)。②光学显微镜下,右侧动脉内膜增厚及脂质斑块情况轻于左侧。通心络组内膜增生相对较轻,斑块较小。通心络组动脉内膜面积(I)/中膜面积(M)为0.47±0.06,显著低于对照组的1.16±0.09(P<0.01)。③通心络组CD40信号强度为0.17±0.04,显著低于对照组的0.36±0.07(P<0.01)。结论去除外膜合并高脂饮食可成功复制兔动脉粥样硬化模型;通心络具有抗动脉粥样硬化作用,其机制可能与降血脂和下调CD40表达有关。Objective To evaluate the influence of Tongxinluo on formation of vascular adventitia damageinduced atherosclerotic plaques and CD40 expression in rabbits. Methods Sixteen male rabbits with left carotid adventitia removed were fed with high cholesterol diet and were divided into two groups randomly：one group was lavaged with Tongxinluo（0. 3 g · kg^-1 · d^-1 ） and the other with water. The bilateral carotid artery specimens and blood samples were collected 12 weeks later. The plaque areas were morphologically determined and the expression of CD40 was detected immunohistochemically. Results There was no difference in total cholesterol, TG, and LDL-C between the 2 groups before experiment （P 〉 0. 05）. The contents of TC and LDL-C were significantly lower in Tongxinluo group than in the control group （both P 〈 0.05） ; the contents of TG were similar between the 2 groups （P 〉 0.05）. The plaque in the right side was slighter than that in left side under light microscope. The hyperplasia of intima in Tongxinluo group was slighter and the plaque was small. The intima（I）/media（M） of artery in Tongxinluo group was 0. 47 ± 0.06, significantly lower than that of the control group（1. 16 ± 0. 09, P 〈 0.01）. The expression of CD40 in Tongxinluo group was significantly lower than that of the control group （0.17±0.04 vs 0. 36 ± 0. 07, P 〈 0.01）. Conclusion Carotid atherosclerosis model can be created by removing arterial adventitia combined with high fat diet in rabbits. Tongxinluo has anti-atherosclerosis effect and the mechanism is possibly related to down-regulaton of blood lipid and expression of CD40. （Shanghai Med J, 2007, 30：734-736）

关 键 词：血管外膜 动脉粥样硬化 通心络 炎症 免疫组织化学 CD40 

分 类 号：R285.5[医药卫生—中药学]