高通量GLGI鉴定及分析白念珠菌LongSAGE标签  

Identification and Analyses of LongSAGE Tags of Candida Albicans by High-throughput GLGI

作　　者：闫洁[1] 周村建[1] 郝飞[1] 

机构地区：[1]第三军医大学西南医院皮肤科,重庆400038

出　　处：《中国皮肤性病学杂志》2007年第10期595-598,共4页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助项目(No.30200013)

摘　　要：目的鉴定并初步分析白念珠菌长片段基因表达序列分析(LongSAGE)标签。方法建立了一种高通量鉴定LongSAGE标签的技术,应用该技术可批量扩增白念珠菌LongSAGE标签,使其由17bp扩增至相应的3'cDNA末端,扩增产物TA克隆后进行测序及序列分析(GLGI)。结果30条多重匹配标签中28条得到稳定有效的扩增,40个无匹配标签中30条得到有效扩增,其中21条证实为新表达序列标签(EST),可能代表新的基因,9条和已知基因有一定同源性,可能是已知基因或其同一家族基因。结论应用高通量GLGI技术成功鉴定了白念珠菌LongSAGE标签,为进一步研究白念珠菌菌相转换机制奠定了基础。To identify and analyze the LongSAGE tags of Candida albicans. Methods We have improved a new technique called high-throughput generation of longer cDNA fragments from serial analysis of gene expression （SAGE） tags for gene identification （GLGI） which could convert LongSAGE tags from 17 bases into their corresponding 3^＋ cDNA fragments simultaneously. The PCR amplified DNA fragments were TA-cloned and screened, and the sequences were analyzed. Results 28 of 30 matched tags were stably and efficiently extended, and 30 of 40 unmatched tags were extended. Among the total,21 tags were confirmed as novel 3 EST, and 9 were reclassified as known sequences because they had certain homology with known genes. Conclusion LongSAGE tags of Candida albicans were successfully identified by applying this high-throughput procedure, which established a basis for further investigating the mechanism of morphism transformation of Candida albicans.

关 键 词：GLGI LonsSAGE 白念珠茵 

分 类 号：R379.4[医药卫生—病原生物学]