环境扫描电镜在牙本质湿黏结界面观察中的应用  

Application of ESEM in the Observation for Moist Dentin Samples

作　　者：王林芳[1] 邝容[2] 倪龙兴[2] 

机构地区：[1]陕西师范大学化学与材料科学学院,710062 [2]第四军医大学口腔医学院,陕西西安710032

出　　处：《实验室研究与探索》2007年第10期36-37,97,共3页Research and Exploration In Laboratory

摘　　要：用环境扫描电镜低真空模式观察新鲜制备的牙本质湿黏结样本。通过对条件的比较，发现在样品室气压1．19×10^2～1．30×10^2Pa，加速电压15kV，柬斑4．0，工作距离8．5～11mm这一条件下，牙本质湿黏结样本变化小，充放电现象少，能获得满意的结果。Moist dentin samples with different characteristics were observed in the environmental scanning electron microscope （ESEM）. The optimal low vacuum condition for moist dentin samples was determined by changing the combination of the chamber pressure, high voltage （HV）, work distance （WD） and spot size. When the chamber pressure at 1.19 × 10^2 ～1.30× 10^2 Pa respectively, HV is 15 kV, spot size is 4.0, and WD is about 10 mm（8.5 ～11 mm）, the moist dentin samples will be shown completely exposed without dehydration, nor obvious charging artifact.

关 键 词：环境扫描电镜 低真空模式 牙本质 

分 类 号：Q336[生物学—遗传学]