兔缺血再灌注心肌的蛋白质组学双向凝胶电泳分析  

Proteomic Analysis of Ischemic-reperfusion Rabbit Myocardium with Two Dimensional Polyacrylamide Gel Electrophoresis

作　　者：冉珂[1] 邹定全[1] 周建美[1] 常业恬[1] 

机构地区：[1]中南大学湘雅二医院麻醉科,湖南长沙410011

出　　处：《医学临床研究》2007年第10期1643-1646,共4页Journal of Clinical Research

基　　金：湖南省自然科学基金项目(NO.03JJY3053)

摘　　要：【目的】研究缺血再灌注心肌的相关蛋白变化。【方法】将8只新西兰大白兔随机分为2组(n=4):正常心肌对照组(C组)和缺血再灌注心肌组(R组)。C组正常灌注160 min,不阻断左冠状动脉;R组左冠状动脉前降支阻断40 min,开放再灌注120 min。分别取各组左心室心肌进行二维凝胶电泳,利用ImageMaster 2D软件分析实验结果。【结果】R组和C组对比,有17个蛋白表达发生了显著变化,其中表达增强的有7个蛋白,表达降低的有4个蛋白,表达不匹配(仅在R组出现)的有6个蛋白点。【结论】这些差异表达的蛋白可能在心肌缺血再灌注损伤的保护中发挥作用。[Objective]To analyze the expression of proteome in myocardial tissue of ischemic-reperfusion rabbit. [Methods] Eight New Zealand white rabbits of both sexes were randomly assigned to group C and group R. Group R underwent ligation of the left anterior descending coronary artery （LAD） for 40 minutes and reperfusion for 120 minutes. Group C kept flowing for 160 minutes without ischemic-reperfusion. Two dimensional polyacrylamide gel electrophoresis（2-DE） was followed by computer assisted image analysis and used to screen changes in protein patterns of extract of myocardium from Group C and Group R. [Results] Compared with Group C, there were 17 protein spots displayed quantitative changes in expression in Group R（P〈0, 05）, of which, 7 spots showed high expression , 4 spots decreased in abundance and 6 only expressed in group R. [Conclusion] These differential proteins may play an important role in protection of myocardial ischemic-reperfusion injury.

关 键 词：心肌 蛋白质类/分析 再灌注损伤 电泳 凝胶 双向 

分 类 号：R446.112[医药卫生—诊断学]