纳米组织工程骨对骨缺损修复区骨密度及生物力学的影响  

The Experimental Study on Bone Mineral Density and Biomechanics in Bone Defects after the Application of the Nano Tissue-Engineered Bone

作　　者：王明海[1] 董有海[1] 洪洋[1] 黄汉伟[1] 顾军[1] 吴俊国[1] 王晓平[1] 童文卿[1] 钱光[1] 何军[1] 

机构地区：[1]复旦大学附属上海市第五人民医院骨科,上海200240

出　　处：《中国临床医学》2007年第5期678-680,共3页Chinese Journal of Clinical Medicine

基　　金：上海市科委科技攻关计划(064119649);复旦大学青年科学基金项目(JKF161001)

摘　　要：目的:将纳米组织工程骨植入兔桡骨骨缺损区,通过对骨缺损区修复后骨组织骨密度和生物力学的研究,揭示其对成骨的影响。方法:制作兔骨缺损动物模型并于缺损区应用纳米组织工程骨。实验兔分为4组:A组为空白对照,B组为单纯纳米相羟基磷灰石胶原复合材料(NHAC),C组为成骨细胞+NHAC,D组为成骨细胞+血管内皮细胞+NHAC。12周后取骨缺损区标本,通过骨密度和生物力学测定,观察成骨情况。结果:D组骨缺损区修复后骨组织骨密度和生物力学测定结果显示新生骨质量较高,骨愈合情况要优于其他组。结论:纳米组织工程骨应用于骨缺损修复可促进骨生成,且成骨细胞+血管内皮细胞+NHAC的效果较好。Objective： To explore the osteogenesis in bone defects after the application of the nano tissue engineered bone by the study of bone mineral density examination and biomechanics examination. Methods： The rabbits radio bone defect model was made and repaired with the the nano tissue-engineered bone. Four groups were divided into Group A, treated with nothing; Group B, treated with nano HAP/Collagen（NHAC） ; Group C, treated with NHAC which co-cultured with osteoblast and Group D, treated with NHAC which co-cultured with osteoblast and vascular endothelial cells; Observed the osteogenesis in bone defects by bone mineral density examination and biomechanics examination in the 12 weeks after treating. Results： D groups＇ quality of regeneration of new bone was better than the others by bone mineral density examination and biomechanics examination. Conclusion： The nano tissue engineered bone can accelerate the osteogenesis and bone restoration. The nano tissueengineered bone which co-cultured with osteoblast and vascular endothelial cells excelled the others.

关 键 词：骨髓基质干细胞 成骨细胞 血管内皮细胞 组织工程 纳米 骨密度 生物力学 

分 类 号：R687.34[医药卫生—骨科学]