PRV、PPV双重PCR检测方法的建立及其应用  

The Establishment and Application of a Double PCR for PRV and PPV

作　　者：曲光刚[1] 沈志强[1] 管宇[1] 王金良[1] 唐娜[1] 谢金文[1] 

机构地区：[1]山东省滨州畜牧兽医研究院,山东滨州256600

出　　处：《中国动物检疫》2007年第11期22-23,共2页China Animal Health Inspection

摘　　要：为建立一种能够快速诊断猪伪狂犬病毒(PRV)、猪细小病毒(PPV)的方法,根据GenBank上发表的PRV、PPV核苷酸序列分别设计并合成了两对能特异性扩增PRV、PPV的引物,经过条件优化后,建立检测PRV、PPV的双重PCR方法,扩增两种病毒的片段分别为570bp、748bp。试验证明,该方法具有良好的特异性和敏感性,省时、省力、快速、敏感、高效的特点,为PRV与PPV的快速诊断试剂盒组装及流行病学的研究提供了技术支持。According to the nucleotide sequences pseudorabies virus （PRV） and porcine parvoviruses （PPV） in Gen- Bank,two pairs of primers were designed and synthesized.A double PCR was developed based on the two primer pairs of primers which amplified the PRV virus-specific segment with the size 570bp and the PPV virus-specific segment with the size 748bp after the PCR conditions were optimized. The results indicated that the double PCR show a good foundation of sensitive and special assay for the clinical diagnosis and epidemiological research.

关 键 词：双重PCR 猪伪狂犬病毒 猪细小病毒 

分 类 号：S858.23[农业科学—临床兽医学]