大豆子叶节、胚尖再生植株的研究  

PLANT REGENERATION FROM COTYLDEONARY NODES AND EMBRYONIC TIPS OF SOYBEAN

作　　者：李海燕[1] 武小霞[1] 刘淼[1] 韩英鹏[1] 

机构地区：[1]东北农业大学大豆研究所教育部大豆生物学重点实验室,哈尔滨150030

出　　处：《大豆科学》2007年第5期709-712,共4页Soybean Science

基　　金：大豆生物学教育部重点实验室主任基金项目(SB07A04)

摘　　要：为了获得高效的大豆再生体系,以大豆品种合丰35和北京小黑豆的成熟种子为材料,利用氯气和升汞两种消毒方法,研究不同浓度6-BA和2,4-D对子叶节和茎出芽的影响。结果表明:在大豆萌发时,加入1.0 mg L^(-1)6-BA和2.0 mg L^(-1)2,4-D,每个外植体分化的芽数较高;在茎尖再生系统中,6-BA和2,4-D诱导茎尖不定芽形成的最佳浓度是3.0 mg L^(-1)和3.5 mg L^(-1),最佳诱导时间是24-48 h。In order to obtain high efficiency soybean regeneration system,cotyledonary nodes and stem tips of soybean Hefeng 35 and Peking was used as explants. The disinfeet ways of mereuric chloride and chlorine gas were compared,and the effeet of 6-BA and 2,4-D on the regeneration of soybean cotyledonary node and stem tip were investigated. The results showed chlorine gas was a better disinfection method. The best concentration of 6-BA and 2,4-D was 1.0and 2.0 mg L^-1 in germination medium respectively. The optimum concentration of 6-BA and 2,4-D was 3.0 and 3.5 mg L^-1 in regeneration of stem tips. And the best inducing time was 24-28 h.

关 键 词：大豆 子叶节 茎尖 激素 再生 

分 类 号：S565.1[农业科学—作物学]