HPLC法测定米格列奈含量及有关物质  

Determination of Mitiglinide and its related substances by HPLC

作　　者：林志立[1] 

机构地区：[1]莆田学院医学院,莆田351100

出　　处：《海峡药学》2007年第10期46-48,共3页Strait Pharmaceutical Journal

摘　　要：目的建立米格列奈含量及有关物质的HPLC测定方法。方法以C18柱，乙腈-水（60：40，用磷酸调pH值至3．0）为流动相。流速为1．0mL·min^-1，柱温25℃，检测波长为210nm。结果在浓度为39．16～352．4；xg范围线形关系良好，相关系数r=0．9999（n=5）。重复性试验的RSD为0．7％（n=6）。有关物质分离较好。结论本方法简单、准确，可作为米格列奈含量和有关物质的测定方法。OBJECTIVE To develop a HLPC method for the determination of Mitiglinide and its related substances. METHODS The determination was carried out on a C18 column. The mobile phase was consisted of acetonitrile-water（40：60 ,pH = 3.0 with phosopodc acid）. The detection wave-length was 210nm. RESULTS There was a good linearity overthe range of 39.16 - 352.4 μg with a correlation coefficient r = 0. 9999 （ n = 5）. The RSD for repetition was 0.7% （n = 6）. CONCLUSION The method was simple and accurate, it can be used as a method for the determination of Mi tiglinide and its related substances.

关 键 词：米格列奈 含量测定 有关物质 HPLC法 

分 类 号：R927.2[医药卫生—药学]